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Biocompatibility Assessment of SiC Surfaces After Functionalization with Self Assembled Organic Monolayers

Published online by Cambridge University Press:  31 January 2011

Alexandra Oliveros ,
Sebastian J. Schoell ,
Christopher Frewin ,
Marco Hoeb ,
Martin Stutzmann ,
Ian D. Sharp  and
Stephen E. Saddow
Alexandra Oliveros
Affiliation:
amolive4@mail.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Sebastian J. Schoell
Affiliation:
sebastian.schoell@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Christopher Frewin
Affiliation:
hlodyn676@msn.com, University of South Florida, Electrical Engineering, Tampa, Florida, United States
Marco Hoeb
Affiliation:
Marco.Hoeb@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Martin Stutzmann
Affiliation:
Stutz@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Ian D. Sharp
Affiliation:
ian.sharp@wsi.tum.de, Walter Schottky Institut, Technische Universität München, Garching, Germany
Stephen E. Saddow
Affiliation:
saddow@eng.usf.edu, University of South Florida, Electrical Engineering, Tampa, Florida, United States
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Abstract

The biocompatibility of 6H-SiC (0001) surfaces was increased by more than a factor of six through the covalent grafting of NH2 terminated self-assembled monolayers (SAM) using APDEMS and APTES molecules. Surface functionalization began with a hydroxyl, OH, surface termination. The study included two NH2 terminated surfaces obtained through silanization with APDEMS (aminopropyldiethoxymethylsilane) and APTES (aminopropyltriethoxysilane) molecules (hydrophilic surfaces) and a CH3 terminated surface produced via alkylation with 1-octadecene (hydrophobic surface). H4 human neuroglioma and PC12 rat pheochromocytoma cells were seeded on the functionalized surfaces and the cell morphology was evaluated with atomic force microscopy (AFM). In addition, 96 hour MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays were employed to evaluate the cell viability on the SAM modified samples. The biocompatibility was enhanced with a 2 fold (171-240%) increase with 1-octadecene, 3-6 fold (320-670%) increase with APDEMS and 5-8 fold (476-850%) increase with APTES with respect to untreated 6H-SiC surfaces.

Keywords

biomaterialself-assemblyscanning probe microscopy (SPM)
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1235: Symposium RR – Engineering Biomaterials for Regenerative Medicine , 2009 , 1235-RR03-43
Copyright
Copyright © Materials Research Society 2010

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