Skip to main content Accessibility help

Antitumoral drug loaded in TEOS nanoparticles

  • Ana Paula V. Araújo (a1), Claure N. Lunardi (a1) and Anderson J. Gomes (a1)


Methotrexate (MTX), is a potent immunomodulating drug and widely used in the treatment of cancer, psoriasis and others disease. Despite its efficacy, the use of MTX is greatly limited due to its toxicity. To solve this problem, we prepared nanoparticles of tetraethyl orthosilicate (NP-TEOS) containing the compound methotrexate (MTX), by the sol-gel method. This drug delivery system (DDS) showed a loading efficiency of 39.7%. Size distribution studies were performed with dynamic light scattering and scanning electron microscopy revealing that these particles were spherical in shape, with a mean diameter between 140-430 nm and a low polydispersity (0.12 – 0.26). Also the particles displayed a low tendency toward aggregation which was confirmed by the low zeta potential -61.4 mV. Profile release showed a slow release loaded with MTX (PBS buffer pH = 7.4). The slow release can be attributed to the low porosity of the NP-TEOS and the extremely low diffusivity of MTX in aqueous media. B16-F10 cells were used to assay the toxicity and uptake of NP-TEOS showing to be nontoxic without MTX making a good candidate for DDS.



Hide All
1. Barbe, C., Bartlett, J., Kong, L. G., Finnie, K., Lin, H. Q., Larkin, M., Calleja, S., Bush, A., and Calleja, G., Advanced Materials, 16, 1959 (2004).
2. Du, L., Song, H. Y., and Liao, S. J., Microporous and Mesoporous Materials, 147, 200 (2012).
3. Bitar, A., Ahmad, N. M., Fessi, H., and Elaissari, A., Drug Discovery Today, 17, 1147 (2012).
4. Lee, J. E., Lee, D. J., Lee, N., Kim, B. H., Choi, S. H., and Hyeon, T., Journal of Materials Chemistry, 21, 16869 (2011).
5. Kenzaoui, B. H., Bernasconi, C. C., Guney-Ayra, S., and Juillerat-Jeanneret, L., Biochemical Journal, 441, 813 (2012).
6. Zhai, W. Y., He, C. L., Wu, L., Zhou, Y., Chen, H. R., Chang, J., and Zhang, H. F., Journal of Biomedical Materials Research Part B-Applied Biomaterials, 100B, 1397 (2012).
7. DeMuth, P., Hurley, M., Wu, C. W., Galanie, S., Zachariah, M. R., and DeShong, P., Microporous and Mesoporous Materials, 141, 128 (2011).
8. Sekine, I., Fukuda, H., Kunitoh, H., and Saijo, N., Japanese Journal of Clinical Oncology, 28, 463 (1998).
9. Gao, K. P. and Jiang, X. G., International Journal of Pharmaceutics, 310, 213 (2006).
10. Seo, D. H., Jeong, Y. I., Kim, D. G., Jang, M. J., Jang, M. K., and Nah, J. W., Colloids and Surfaces B-Biointerfaces, 69, 157 (2009).
11. Mashhadi, M. A., Iranian Red Crescent Medical Journal, 10, 75 (2008).
12. Tang, W., Xu, H., Kopelman, R., and Philbert, M. A., Photochemistry and Photobiology, 81, 242 (2005).
13. Diab, R., Jaafar-Maalej, C., Fessi, H., and Maincent, P., Aaps Journal, 14, 688 (2012).
14. Ivanov, S., Zhuravsky, S., Yukina, G., Tomson, V., Korolev, D., and Galagudza, M., Materials, 5, 1873 (2012).
15. Yildirimer, L., Thanh, N. T. K., Loizidou, M., and Seifalian, A. M., Nano Today, 6, 585 (2011).
16. Albarran, L., Lopez, T., Quintana, P., and Chagoya, V., Colloids and Surfaces A-Physicochemical and Engineering Aspects, 384, 131 (2011).
17. Andhariya, N., Chudasama, B., Mehta, R. V., and Upadhyay, R. V., Journal of Nanoparticle Research, 13, 3619 (2011).
18. Gomes, A. J., Lunardi, L. O., Caetano, F. H., Machado, A. E. H., Oliveira-Campos, A. M. F., Bendhack, L. M., and Lunardi, C. N., Journal of Applied Polymer Science, 121, 1348 (2011).
19. Kumar, R., Roy, I., Ohulchanskky, T. Y., Vathy, L. A., Bergey, E. J., Sajjad, M., and Prasad, P. N., Acs Nano, 4, 699 (2010).
20. Gomes, A. J., Lunardi, C. N., and Tedesco, A. C., Photomedicine and Laser Surgery, 25, 428 (2007).
21. Gomes, A. J., Lunardi, C. N., Lunardi, L. O., Pitol, D. L., and Machado, A. E. H., Micron, 39, 40 (2008).
22. Lorenz, M. R., Holzapfel, V., Musyanovych, A., Nothelfer, K., Walther, P., Frank, H., Landfester, K., Schrezenmeier, H., and Mailander, V., Biomaterials, 27, 2820 (2006).
23. Gomes, A. D., Lunardi, C. N., Caetano, F. H., Lunardi, L. O., and Machado, A. E. D., Microscopy and Microanalysis, 12, 399 (2006).
24. Lee, C. H., Lo, L. W., Mou, C. Y., and Yang, C. S., Advanced Functional Materials, 18, 3283 (2008).



Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed