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Use of Soft Lithography for Multi-layer MicroMolding (MMM) of 3-D PCL Scaffolds for Tissue Engineering

Published online by Cambridge University Press:  15 March 2011

Yang Sun ,
Nicholas Ferrell  and
Derek J. Hansford
Yang Sun
Affiliation:
Biomedical Engineering Center, The Ohio State University, 270 Bevis Hall, 1080 Carmack Rd., Columbus, OH 43210, U.S.A.
Nicholas Ferrell
Affiliation:
Biomedical Engineering Center, The Ohio State University, 270 Bevis Hall, 1080 Carmack Rd., Columbus, OH 43210, U.S.A.
Derek J. Hansford
Affiliation:
Biomedical Engineering Center, The Ohio State University, 270 Bevis Hall, 1080 Carmack Rd., Columbus, OH 43210, U.S.A.
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Abstract

Tissue engineering scaffolds with precisely controlled geometries, particularly with surface features smaller than typical cell dimensions (1-10μm), can improve cellular adhesion and functionality. In this paper, soft lithography was used to fabricate polydimethylsiloxane (PDMS) stamps of arrays of parallel 5μm wide, 5μm deep grooves separated by 45 μm ridges, and an orthogonal grid of lines with the same geometry. Several methods were compared for the fabrication of 3-D multi-layer polycaprolactone (PCL) scaffolds with precise features. First, micromolding in capillaries (MIMIC) was used to deliver the polymer into the small grooves by capillarity; however the resultant lines were discontinuous and not able to form complete lines. Second, spin coating and oxygen plasma were combined to build 3-D scaffolds with the line pattern. The resultant scaffolds had good alignment and adhesion between layers; however, the upper layer collapsed due to the poor mechanical rigidity. Finally, a new multi-layer micromolding (MMM) method was developed and successfully applied with the grid pattern to fabricate 3-D scaffolds. Scanning electron microscopy (SEM) characterization showed that the multi-layered scaffolds had high porosity and precisely controlled 3-D structures.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 820: Symposia O/R – Nanoengineered Assemblies and Advanced Micro/Nanosystems , 2004 , O5.3/W9.3
Copyright
Copyright © Materials Research Society 2004

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References

1. Curtis, A., Riehle, M.. Phys. Med. Biol. 46, R47 (2001).Google Scholar
2. Vats, A., Tolley, N.S., Polak, J.M., J.E., Clin. Otolaryngol. 28(3), 165 (2003).Google Scholar
3. Hutmacher, D.W.. Biomaterials 21, 2529 (2000).Google Scholar
4. Curtis, A., Wilkinson, C.. Biomaterials 18(24), 1573 (1997).Google Scholar
5. Freed, L.E., Marquis, J.C., Vunjak-Novakovic, G., Emmanual, J., Langer, R.. Biotechnol. Bioeng. 43, 605 (1994).Google Scholar
6. Ma, T., Li, Y., Yang, S.T.. Biotechnol. Prog. 15, 715 (1999).Google Scholar
7. Walboomers, X.F., Jansen, J.A.. Ondotology 89, 2 (2001).Google Scholar
8. Flemming, R.G., Murphy, C.J., Abrams, G.A., Goodman, S.L., Nealey, P.F.. Biomaterials 20, 573 (1999).Google Scholar
9. Ito, Y.. Biomaterials 20, 2333 (1999).Google Scholar
10. Curtis, A.S.G., Varde, M.. J. Nat. Cancer. Res. Inst. 33, 15 (1964).Google Scholar
11. Bhatia, S.N., Chen, C.S.. Biomed. Microdevices 2(2), 131(1999).Google Scholar
12. Recum, A.F. von, Kooten, T.G. van. J. Biomater. Sci. Polym. Ed. 7, 181 (1995).Google Scholar
13. Brunette, D.M., Kenner, G.S., Gould, T.R.L.. J. Dent. Res. 62, 1045 (1983).Google Scholar
14. Clark, P.. Biosens. Bioelec. 9, 657 (1994).Google Scholar
15. Dalby, M.J., Riehle, M.O., Yarwood, S.J., Wilkinson, C.D.W., Curtis, A.S.G.. Experimental Cell Research 284, 274 (2003).Google Scholar
16. Folch, A., Mezzour, S., During, M., Hurtado, O., Toner, M., Muller, R.. Biomed. Microdevices 2, 207 (2000).Google Scholar
17. Desai, T.A.. Medical Engineering & Physics 22, 595 (2000).Google Scholar
18. Vozzi, G., Flaim, C., Ahluwalia, A., Bhatia, S.. Biomaterials 24, 2533 (2003).Google Scholar
19. Ward, J.H., Bashir, R., Peppas, N.A.. J. Biomed. Mater. Res. 56, 351 (2001).Google Scholar
20. Liu, V.A., Bhatia, S.N.. Biomed. Microdevices. 4, 257 (2002).Google Scholar
21. Yan, Y., Xiong, Z., Hu, Y., Wang, S., Zhang, R., Zhang, C.. Materials Letters 57, 2623 (2003).Google Scholar
22. Janshoff, A., Künneke, S.. European Biophysics Journal 29, 549 (2000).Google Scholar
23. Li, S.H., Wijn, J.R. De, Layrolle, P., Groot, K. de. J. Biomed. Mater. Res. 61, 109 (2002).Google Scholar