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Protein-Directed Self-Assembly of Gold Nanoparticles

Published online by Cambridge University Press:  26 February 2011

Alexey Vertegel ,
Wen Shang ,
Jonathan Dordick  and
Richard Siegel
Alexey Vertegel
Affiliation:
vertege@clemson.edu, Clemson University, Bioengineering, 401 Rhodes Hall, Clemson, SC, 29634-0905, United States, 864-656-0801, 864-656-4466
Wen Shang
Affiliation:
shangw@rpi.edu, Rensselaer Polytechnic Institute, United States
Jonathan Dordick
Affiliation:
dordick@rpi.edu, Rensselaer Polytechnic Institute
Richard Siegel
Affiliation:
rwsiegel@rpi.edu, Rensselaer Polytechnnic Institute
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Abstract

We have employed protein-protein interactions for controlled assembly of gold nanoparticles. Stoichiometric 1:1 protein:nanoparticle conjugates were prepared for proteins known to strongly interact with each other and these interactions were used to self-assemble nanoparticles. Mixing equivalent amounts of the antigen-nanoparticle and antibody-nanoparticle conjugates resulted in the formation of nanoparticle dimers with a yield of about 60%. Trimers (yield ∼30%) can be obtained by mixing 2:1 antigen-nanoparticle with 1:1 antibody-nanoparticle conjugates in a molar ratio of 1:2. The structures are destroyed at low pH when the antibody-antigen complex dissociates.

Keywords

biomimetic (assembly)nanostructureAu
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 901: Symposium R – Assembly at the Nanoscale – Toward Functional Nanostructured Materials , 2005 , 0901-Ra10-06
Copyright
Copyright © Materials Research Society 2006

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