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Insertion of Inorganic-Biomolecular Nanohybrid into Eucaryotic Cell

Published online by Cambridge University Press:  15 March 2011

Seo-Young Kwak
Affiliation:
School of Chemistry & Molecular Engineering, Seoul National University, Seoul 151-747, KOREA
Sung-Ho Hwang
Affiliation:
School of Chemistry & Molecular Engineering, Seoul National University, Seoul 151-747, KOREA
Yong-Joo Jeong
Affiliation:
School of Chemistry & Molecular Engineering, Seoul National University, Seoul 151-747, KOREA
Jong-Sang Park
Affiliation:
School of Chemistry & Molecular Engineering, Seoul National University, Seoul 151-747, KOREA
Jin-Ho Choy
Affiliation:
School of Chemistry & Molecular Engineering, Seoul National University, Seoul 151-747, KOREA
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Abstract

It has been clearly demonstrated that ATP could be intercalated into inorganic layered double hydroxide (LDH), giving rise to a biomolecular-inorganic nanohybrid with preserving its physico-chemical and biological integrity. It shows a remarkable transfer efficiency of ATP into target cells by alleviating an electrical repulsion at the cell walls due to the neutralization of negative charge of phosphates by positive hydroxide layers. From cellular uptake experiment with laser scanning confocal fluorescence microscopy, it is revealed that the FITC-LDH hybrid is effectively transferred into 293 cells. Such an unique feature of biomolecule-LDH hybrid will open a new field of reserving and delivering genes, drugs and other functional biomolecules.

Type
Research Article
Copyright
Copyright © Materials Research Society 2002

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References

REFERENCES

1. Choy, J. H., Kwak, S. Y., Park, J. S., and Jeong, Y. J., J. Mater. Chem., 11(6), 16711674 (2001)Google Scholar
2. Choy, J. H., Kwak, S. Y., Jeong, Y. J., and Park, J. S., Angew. Chem. Int. Ed. as a highlight comm. 39(22), 40424045 (2000)Google Scholar
3. Wickstrom, E. L., Bacon, T. A., Gonzalez, A., Freeman, D. L., Lyman, G. H., Wickstrom, E., Proc. Natl. Acad. Sci. USA 85, 1028 (1988).Google Scholar
4. Lee, R. J. and Low, P. S., J. Biol. Chem. 269, 31983204 (1997).Google Scholar
5. Lee, Z. W. et al. Ibid. 273, 1271012715 (1998).Google Scholar
6. Vogel, K., Wang, S., Lee, R. J., Chmielewski, J., Low, P. S., J. Am. Chem. Soc. 118, 15811586 (1996).Google Scholar
7. Hansen, M. B., Nielsen, S. E., Berg, K., J. Immunol. Methods 119, 201203 (1989).Google Scholar
8. Choy, J. H., Kwak, S. Y., Park, J. S., Jeong, Y. J., and Portier, J., J. Am. Chem. Soc., 121, (1999)13991400.Google Scholar
9. Davis, S. S., Trends Biotechnol. 15, 217 (1997).Google Scholar
10. Verma, I. M., Somia, N., Nature 389, 239 (1997).Google Scholar
11. Bennet, C. F., Chiang, M. Y., Chan, H., Shoemaker, J. E. E., Mirabelli, C. K., Pharmacol. 41, 1023 (1992).Google Scholar
12. Wu, G. Y., Wu, C. H., J. Biol. Chem. 11, 1050 (1987).Google Scholar
13. Boussif, O., Lesoulac'h, F., Zanta, M. A., Mergny, M. D., Scherman, D., emeneix, B., Behr, J. P., Proc. Natl. Acad. Sci. USA 92, 7297 (1995).Google Scholar
14. Zanta, M. A., Boussif, O., Adib, A., Behr, J. P., Bioconjugate Chem. 8, 839 (1997).Google Scholar
15. Choksakulnimitr, S., Masuda, S., Tokuda, H., Takakura, Y., Hashida, M., J. Controlled Release 34, 233 (1995).Google Scholar
16. Brazeau, G. A., Attia, S., Poxon, S., Hughes, J. A., Pharm. Res. 15, 680 (1998).Google Scholar
17. Putnam, D., Langer, R., Macromolecules 32, 3658 (1999).Google Scholar
18. Judith, K. G. C., Bodepudi, V., Bishop, J. S., Jayaraman, K., Chaudhary, N., J. Biol. Chem. 270, 31391 (1995).Google Scholar