Skip to main content Accessibility help
×
Home
Hostname: page-component-768dbb666b-l8xdn Total loading time: 0.305 Render date: 2023-02-02T18:16:03.438Z Has data issue: true Feature Flags: { "useRatesEcommerce": false } hasContentIssue true

Effects on Hardness and Elastic Modulus for DSS-8 Peptide Treatment on Remineralization of Human Dental Tissues

Published online by Cambridge University Press:  15 March 2011

Chia-Chan Hsu
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
Hsiu-Ying Chung
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A. Department of Materials Science and Engineering, Feng Chia University, Taichung 407, Taiwan, Republic of China
Elizabeth Marie Hagerman
Affiliation:
Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
Wenyuan Shi
Affiliation:
School of Dentistry, University of California, Los Angeles, CA 90095, U.S.A.
Jenn-Ming Yang
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
Ben Wu
Affiliation:
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A. Department of Bioengineering, University of California, Los Angeles, Los Angeles, CA 90095, U.S.A.
Get access

Abstract

Dental remineralization may be achieved by mediating the interactions between tooth surfaces with free ions and biomimetic peptides. We recently developed octuplet repeats of aspartate-serine-serine (DSS-8) peptide, which occurs in high abundance in naturally occurring proteins that are critical for tooth remineralization. In this paper, we evaluated the possible role of DSS-8 in enamel remineralization. Human enamel specimens were demineralized, exposed briefly to DSS-8 solution, and then exposed to concentrated ionic solutions that favor remineralization. Enamel nano-mechanical behaviors, hardness and elastic modulus, at various stages of treatment were determined by nanoindentation. The phase, microstructure and morphology of the resultant surfaces were characterized using the grazing incidence X-ray diffraction (GIXD), variable pressure scanning electron microscopy (VPSEM), and atomic force microscopy (AFM), respectively. Nanoindentation results show that the DSS-8 remineralization effectively improves the mechanical and elastic properties for demineralized enamel.

Type
Research Article
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Landis, WJ, Song, MJ, Leith, A, McEwen, L, McEwen, BF. Mineral and organic matrix interaction in normally calcifying tendon visualized in three dimensions by high-voltage electron microscopic tomography and graphic image reconstruction. J Struct Biol 1993;110:3954.CrossRefGoogle ScholarPubMed
2. Nanci, A. Ten cate's oral histology. St. Louis: Mosby Elsevier;2003.Google Scholar
3. Suga, S, Watabe, N. Hard tissue mineralization and demineralization. Tokyo: Springer; 1992.CrossRefGoogle Scholar
4. Cuy, JL, Mann, AB, Livi, KJ, Teaford, MF, Weihs, TP. Nanoindentation mapping of the mechanical properties of human molar tooth enamel. Arch Oral Biol 2002; 47:281–91.CrossRefGoogle ScholarPubMed
5. Stetler, WGS, Veis, A. Bovine dentin phosphophoryn: calcium ion binding properties of a high molecular weight preparation. Calcif Tissue Int 1987; 40(2):97102.CrossRefGoogle Scholar
6. George, A, Bannon, L, Sabsay, B, Dillon, JW, Malone, J, Veis, A, Jenkins, NA, Gilbert, DJ, Copeland, NG. The carboxyl-terminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J Biol Chem 1996; 271 (51): 32869–73.CrossRefGoogle ScholarPubMed
7. He, G, Dahl, T, Veis, A, Geroge, A. Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1. Nat Mater 2003;2(8):552–8.CrossRefGoogle ScholarPubMed
8. Sikes, CS, Yeung, ML, Wheeler, AP. In surface reactive peptides and polymers: discovery and commercialization. Washington: ACS;1991.CrossRefGoogle Scholar
9. Qiu, SR, Wierzbicki, A, Orme, CA, Cody, AM, Hoyer, JR, Nancollas, GH, Zepeda, S, Yoreo, JJD. Molecular modulation of calcium oxalate crystallization by osteopontin and citrate. Proc Natl Acad Sci 2004;101(7):1811–5.CrossRefGoogle ScholarPubMed
10. Bigi, A, Boanini, E, Rubini, K, Gazzano, M. Hydroxyapatite nanocrystals modified with acidic amino acids. Eur J Inorg Chem 2006;23: 4821–6.Google Scholar
11. Papanearchou, NI, Leventouri, T, Kis, AC, Hotiu, A, Anderson, IM. Effect of simulated body fluid on the microstructure of ferrimagnetic bioglass-ceramics. Mater Res Soc Symp Proc 2005;839:371–6.Google Scholar
12. Kokubo, T, Takadama, H. How useful is SBF in predicting in vivo bone bioactivity. Biomaterials 2006:27:2907.CrossRefGoogle ScholarPubMed
13. Murphy, WL, Monney, DJ. Bioinspired growth of crystalline carbonate apatite on biodegradable polymer substrata. J Am Chem Soc 2002;124(9):1910–7.CrossRefGoogle ScholarPubMed
14. Oliver, WC, Pharr, GM. Review: Measurement of hardness and elastic modulus by instrumented indentation: Advances in understanding and refinements to methodology. J Mater Res 2004;19(1):320.CrossRefGoogle Scholar
15. Zhou, J, Hsiung, LL. Biomolecular origin of the rate-dependent deformation of prismatic enamel. Appl Phys Lett 2006;89:51904.CrossRefGoogle Scholar
16. He, LH, Swain, MV. Influence of environment on the mechanical behavior of mature human enamel. Biomaterials 2007;28:4512–20CrossRefGoogle ScholarPubMed
17. JCPDS, No. 00-001-1008. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar
18. JCPDS, No. 00-035-0180. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar
19. JCPDS, No. 00-004-0697. International Center for Diffraction Data: Newton Square, PA; 2003.Google Scholar

Save article to Kindle

To save this article to your Kindle, first ensure coreplatform@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Effects on Hardness and Elastic Modulus for DSS-8 Peptide Treatment on Remineralization of Human Dental Tissues
Available formats
×

Save article to Dropbox

To save this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Dropbox account. Find out more about saving content to Dropbox.

Effects on Hardness and Elastic Modulus for DSS-8 Peptide Treatment on Remineralization of Human Dental Tissues
Available formats
×

Save article to Google Drive

To save this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you used this feature, you will be asked to authorise Cambridge Core to connect with your Google Drive account. Find out more about saving content to Google Drive.

Effects on Hardness and Elastic Modulus for DSS-8 Peptide Treatment on Remineralization of Human Dental Tissues
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *