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The Combination of Drug or Gene Delivery System Responding to Cellular Signals (D-RECS) and Sonoporation System for Effective and Safe Gene Delivery

Published online by Cambridge University Press:  31 January 2011

Akira Tsuchiya
Affiliation:
akira0813@mail.cstm.kyushu-u.ac.jp, Kyushu University, Faculty of Engineering, Fukuoka, Japan
Takeshi Mori
Affiliation:
mori.takeshi.880@m.kyushu-u.ac.jp, Kyushu University, Faculty of Engineering, Fukuoka, Japan
Yuki Naritomi
Affiliation:
nari-yuki@mail.cstm.kyushu-u.ac.jp, Kyushu University, Faculty of Engineering, Fukuoka, Japan
Jeong-Hun Kang
Affiliation:
jrjhkang@ri.ncvc.go.jp, Kyushu University, Faculty of Engineering, Fukuoka, Japan
Daisuke Asai
Affiliation:
asai@marianna-u.ac.jp, St. Marianna University School of Medicine, Department of Microbiology, Kawasaki, Japan
Takuro Niidome
Affiliation:
niidome.takuro.655@m.kyushu-u.ac.jpniidome-tcm@mbox.nc.kyushu-u.ac.jp, Kyushu University, Faculty of Engineering, Fukuoka, Japan
Yoko Endo
Affiliation:
endo@ps.toyaku.ac.jp, Tokyo University of Pharmacy and Life Sciences, Department of Drug and Gene Delivery System, Hachioji, Japan
Ryo Suzuki
Affiliation:
r-suzuki@pharm.teikyo-u.ac.jp, Teikyo University, Department of Biopharmaceutics, Sagamihara, Japan
Yoichi Negishi
Affiliation:
negishi@ps.toyaku.ac.jp, Tokyo University of Pharmacy and Life Sciences, Department of Drug and Gene Delivery System, Hachioji, Japan
Kazuo Maruyama
Affiliation:
maruyama@pharm.teikyo-u.ac.jp, Teikyo University, Department of Biopharmaceutics, Sagamihara, Japan
Yoshiki Katayama
Affiliation:
ykatatcm@mbox.nc.kyushu-u.ac.jp, Kyushu University, Faculty of Engineering, Fukuoka, Japan
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Abstract

We have developed new gene expression-regulating polymer that can activate transgene expression in response to target intracellular signals. Here, we tried applying sonoporation system to this gene regulation system to enhance the gene expression efficacy. Sonoporation is the method for effective gene transfection in vitro and in vivo. Therefore, the method might enhance the transfection efficiency in our polymer and realize an efficient and safe gene delivery system. Results suggested that the combination of our polymer and sonoporation could improve the gene expression compared to the system using only our polymer that transfers genes into cells via endocytosis. It also kept the ability of the gene regulation responding to cellular signals.

Type
Research Article
Copyright
Copyright © Materials Research Society 2010

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