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Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells

  • Arianna Mauretti (a1) (a2), Fabrizio Rossi (a3), Noortje A. M. Bax (a1) (a2), Carmen Miano (a3), Fabio Miraldi (a4), Marie José Goumans (a5), Elisa Messina (a3) (a6), Alessandro Giacomello (a3), Carlijn V. C. Bouten (a1) (a2) and Cecilia Sahlgren (a1) (a2) (a7) (a8)...
Abstract

Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays a key role in cell fate decisions in the developing and adult heart. Here, we investigated the effect of three-dimensional (3D) spheroid culture, as a model of the 3D microenvironment, on Notch in fetal and adult human CPCs, under room air (20%) and physiological (5%) oxygen tension. Notch signaling is enhanced in 3D spheroids; spheroid culture under 5% O2 further increases Notch signaling enhancement, and might ultimately improve the regenerative potential of CPCs.

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Corresponding author
Address all correspondence to Arianna Mauretti at a.mauretti@tue.nl
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These authors contributed equally to this work.

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References
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MRS Communications
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