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Bioactive paper: Biomolecule immobilization methods and applications in environmental monitoring

Published online by Cambridge University Press:  12 April 2013

Clémence Sicard
Affiliation:
Department of Chemistry and Chemical Biology, McMaster University; csicard@mcmaster.ca
John D. Brennan
Affiliation:
Department of Chemistry and Chemical Biology, McMaster University; brennanj@mcmaster.ca
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Abstract

Bioactive paper, or paper that exhibits biorecognition properties, is a promising tool for on-site environmental monitoring. A critical parameter for production of paper-based sensors is the immobilization of biomolecules onto paper with retention of long-term activity. High loading, control over biomolecule orientation and accessibility, and protection of biomolecules from external denaturation need to be provided by the immobilization technique. We present here a summary of the current strategies for enzyme, antibody, bacteriophage, and aptamer immobilization on cellulose and provide examples of the application of bioactive paper in environmental monitoring.

Type
Research Article
Copyright
Copyright © Materials Research Society 2013

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