To date only few low-cost bio-based materials have been reported to be useful as TENGs. However, they still keep employing costly nanofabrication techniques. Herein, a new bio-based starch-cellulose TENG is fabricated without using complex equipment for the first time. Our results showed that, depending on the film thickness, electric outputs varied from 60 mV to 300 mV per 4 cm2 area. The thicker the film, the lower the electrical outputs, and vice versa. Moreover, FTIR-ATR analysis also showed that no chemical modification was made on the surface of starch after casting. Therefore, starch remained unmodified at the time of characterization, being this performance proper of a pristine starch. In addition, though organic starch isolation, fabrication of the proposed TENG was entirely handmade, thus avoiding use of complex equipment of nano- and micro-fabrication which resulted in the development of an eco-friendly TENG with very good performance according to the state-of-the-art.