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Design of a DNA-Based Biomaterial by Sol-Gel Method: Application for the Recognition of Albendazole Sulfoxide

  • Elani J. Cabrera-Vega (a1), Georgina Alarcón-Ángeles (a1), Martín Gómez Hernández (a1), Gonzalo Campillo-Alvarado (a2) and Marcela Hurtado Y De la Peña (a1)...

Abstract

We describe the use of immobilized deoxyribonucleic acid (DNA) in a silica matrix as a biorecognition agent for the detection of albendazole sulfoxide (ASU), the primary metabolite of albendazole and a suspected teratogenic and embryotoxic agent. The biomaterial (DNA-containing gel) was synthesized by physical entrapment of salmon sperm in an inorganic silicate matrix by the sol-gel method. Functionality of the DNA-containing gel was evaluated by comparative offline frontal chromatography followed by HPLC analysis of ASU and caffeine (CAF, control) using DNA-containing gel and DNA-free gel. The DNA-containing gel showed relatively high specific retention for ASU, while CAF showed no retention using frontal analysis. We anticipate that the DNA-containing gel can be implemented to identify the interactions of DNA with other active pharmaceutical ingredients (APIs) and their metabolites in a readily available, sensitive and selective frontal chromatography experiment.

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Keywords

Design of a DNA-Based Biomaterial by Sol-Gel Method: Application for the Recognition of Albendazole Sulfoxide

  • Elani J. Cabrera-Vega (a1), Georgina Alarcón-Ángeles (a1), Martín Gómez Hernández (a1), Gonzalo Campillo-Alvarado (a2) and Marcela Hurtado Y De la Peña (a1)...

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