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Creating a Novel Graphene Oxide/Iron/Polylactic Acid Composite that Promotes Dental Pulp Stem Cell Proliferation and Mineralization

  • Rebecca Isseroff (a1) (a2), John Chen (a2), Zaiff Khan (a2), Anoushka Guha (a2), Simon Lin (a1), Juyi Li (a1), Kuan-che Fang (a1), Linxi Zhang (a1), Marcia Simon (a3) and Miriam Rafailovich (a1)...

Abstract

Dental pulp stem cells (DPSCs) can differentiate into bone cells when provided the correct environment, potentially generating cells to repair non-union fractures. Polylactic Acid (PLA) is a biocompatible polymer for 3-D printing of scaffolds, but DPSCs do not proliferate well on PLA. With the goal of making PLA more conducive for DPSC growth, Graphene Oxide (GO); partially reduced Graphene Oxide (pRGO); GO with iron nanoparticles (FeGO) or Fe-pRGO were incorporated into PLA and spun cast as thin films onto silicon wafers for DPSC plating. DPSCs on Fe-pRGO displayed the fastest doubling time and the highest cell modulus; Fe-pRGO with exterior magnets produced high cell density. SEM demonstrated DPSC mineralization, whereas PLA-only DPSC cultures showed none. Results suggest that PLA/Fe-pRGO and PLA/pRGO enhance DPSC proliferation and possibly differentiation with the potential for use as a 3-D printed scaffold for tissue engineering.

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References

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