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Zinc Mapping and Density Imaging of Rabbit Pancreas Endocrine Tissue Sections Using Nuclear Microscopy

Published online by Cambridge University Press:  03 July 2009

M.D. Ynsa ,
M.Q. Ren ,
R. Rajendran ,
J.N. Sidhapuriwala ,
J.A. van Kan ,
M. Bhatia  and
F. Watt
M.D. Ynsa*
Affiliation:
Centro de Micro-Análisis de Materiales, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain
M.Q. Ren
Affiliation:
Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore117542
R. Rajendran
Affiliation:
Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore117542
J.N. Sidhapuriwala
Affiliation:
Department of Pharmacology, Cardiovascular Biology Research Group, Yong Loo Lin School of Medicine, National University of Singapore, 18 Medical Drive, Singapore117597
J.A. van Kan
Affiliation:
Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore117542
M. Bhatia
Affiliation:
Department of Pharmacology, Cardiovascular Biology Research Group, Yong Loo Lin School of Medicine, National University of Singapore, 18 Medical Drive, Singapore117597
F. Watt
Affiliation:
Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore117542
*
Corresponding author. E-mail: m.ynsa@uam.es
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Abstract

Nuclear microscopy is a suite of techniques based on a focused beam of MeV protons. These techniques have the unique ability to image density and structural variations in relatively thick tissue sections, map trace elements at the cellular level to the microgram per gram (dry weight) level, and extract quantitative information on these elements. The trace elemental studies can be carried out on unstained freeze-dried tissue sections, thereby minimizing any problems of contamination or redistribution of elements during conventional staining and fixing procedures. The pancreas is a gland with different specialized cells and a complex hormonal activity where trace elements play an important role. For example, zinc has an active role in insulin production, and calcium ions participate in the stimulation and secretion process of insulin. Using nuclear microscopy with a spatial resolution of 1 μm, we have located, using zinc mapping, the islets of Langerhans in freeze-dried normal rabbit tissue sections. The islets of Langerhans contain β-cells responsible for insulin production. Subsequent quantitative analyses have indicated elevations in most elements within the islets of Langerhans, and significantly so for the concentrations of Zn [3,300 compared to 90 μg/g (dry weight)] and Ca [1,100 compared to 390 μg/g (dry weight)].

Keywords

nuclear microprobePIXERBSSTIMZnexocrine pancreasendocrine pancreas
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 15 , Issue 4 , August 2009 , pp. 345 - 352
Copyright
Copyright © Microscopy Society of America 2009

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