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What do we know about stain distribution in cells and tissue? Using EDS to determine the quantity and distribution of common EM stains.

Published online by Cambridge University Press:  30 July 2021

Louise Hughes
Affiliation:
Oxford Instruments NanoAnalysis, United States
Iain Anderson
Affiliation:
Oxford Instruments NanoAnalysis, United States
Errin Johnson
Affiliation:
University of Oxford, United States

Abstract

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Type
To Fix or Not To Fix? A Question for Biological Samples
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Hayat, M. (2000), Principles and techniques of electron microscopy, biological applications. Cambridge University Press, Cambridge.Google Scholar
Kittelmann, M., Hawes, C. and Hughes, L., (2016). Serial block face scanning electron microscopy and the reconstruction of plant cell membrane systems. Journal of microscopy, 263(2), pp.200-211.Google ScholarPubMed
Deerinck, T.J., Bushong, E.A., Thor, A. and Ellisman, M.H., (2010). NCMIR methods for 3D EM: a new protocol for preparation of biological specimens for serial block face scanning electron microscopy. Microscopy, 6(8).Google Scholar
Locke, M., Huie, P. (1977), Bismuth staining for light and electron microscopy, Tissue and Cell, 9 (2) 347-371CrossRefGoogle ScholarPubMed