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Unusual DNA Structures Formed on Bare Highly Oriented Pyrolytic Graphite Surfaces Studied by Atomic Force Microscopy

Published online by Cambridge University Press:  27 March 2013

Zhiguo Liu ,
Lin Zhao ,
Yuangang Zu ,
Shengnan Tan ,
Yuanlin Wang  and
Yiming Zhang
Zhiguo Liu*
Affiliation:
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China
Lin Zhao
Affiliation:
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China
Yuangang Zu*
Affiliation:
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China
Shengnan Tan
Affiliation:
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China
Yuanlin Wang
Affiliation:
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China
Yiming Zhang
Affiliation:
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China
*
*Corresponding author. E-mail: zguoliu@yahoo.com.cn
**Corresponding author. E-mail: nefunano@yahoo.com.cn
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Abstract

It is important to know the detailed DNA structure on carbonaceous surfaces for further application of DNA-functionalized carbonaceous materials in diverse research areas. In this study, the topographic and structural characteristics of the separated single DNA molecules and their assembly on highly oriented pyrolytic graphite (HOPG) surfaces have been investigated by atomic force microscopy (AFM). AFM results indicate that both circular and linear DNA molecules tend to form hexagonal patterns along with some unusual structures that include node, protrusion, cruciform, parallel single-stranded DNA (ssDNA), and compact zigzag. Furthermore, parallel ssDNA patterns and their crossed structures have been obtained under high-temperature conditions. Our AFM results reveal that a bare HOPG surface can induce DNA molecules to form various unusual structures. This finding is helpful for understanding the adsorption behavior of DNA on other carbonaceous surfaces such as carbon nanotubes and graphene. In addition, the hexagonal DNA patterns in this study are similar to those formed on the alkylamine-modified HOPG surface, which implies that a bare HOPG, without any chemical modification, has a strong ability to align biomolecules. This study could expand our knowledge of the diversities of DNA structures and the aligning ability of carbonaceous surfaces.

Keywords

DNAAFMHOPGnodesprotrusions
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 3 , June 2013 , pp. 544 - 552
Copyright
Copyright © Microscopy Society of America 2013 

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