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Ultrastructure and Elemental Distribution in Foliage of Manganese-Treated Sugar Maple Seedlings: Comparison of Freeze-substitution Fixatives

Published online by Cambridge University Press:  02 July 2020

C. J. McQuattie ,
G. A. Schier ,
S. Burns  and
J. W. Heckman
C. J. McQuattie
Affiliation:
USDA Forest Service Research Laboratory, Delaware, OH43015
G. A. Schier
Affiliation:
USDA Forest Service Research Laboratory, Delaware, OH43015
S. Burns
Affiliation:
USDA Forest Service Research Laboratory, Delaware, OH43015
J. W. Heckman
Affiliation:
USDA Forest Service Research Laboratory, Delaware, OH43015
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Extract

Trace metals, such as manganese (Mn) and aluminum (Al), show increased solubility as soil acidity increases. Uptake of these elements may approach toxic concentrations in some plant species growing in acid forest soils. Previously, we had excellent results when chromium trioxide (CrO3) was used as the freeze substitution fixative in examining Al-treated plant and fungal tissue.1-2 However, when the fixative was used to study Mn toxicity, peak overlap (CrK β and MnKα) made it difficult to determine the location of Mn. In contrast, osmium tetroxide (OsO4) has no overlapping peak with Mn and is an excellent freeze substitution fixative. This study compares the use of OsO4 and CrO3 as fixatives for energy dispersive x-ray microanalysis (EDS) of the effect of Mn on ultrastructure and element localization in sugar maple leaves.

Type
Botany/Plant Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1176 - 1177
Copyright
Copyright © Microscopy Society of America

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References

1.McQuattie, C. J., et al., Proc. Ann EMSA Meeting 50(1992)730.Google Scholar
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