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Three-Dimensional Visualization Of Miotic Chromosomes In Maize Trisomy 6

Published online by Cambridge University Press:  02 July 2020

Wayne Y. Cheng ,
Thomas C. Lee ,
David B. Walden  and
Ping-chin Cheng
Wayne Y. Cheng
Affiliation:
Williamsville East HS, Williamsville, NY14051, USA
Thomas C. Lee
Affiliation:
WiIliamsviIle North HS, Williamsville, NY14221, USA
David B. Walden
Affiliation:
Dept. of Plant Sciences, University of Western Ontario, London, Ontario, CanadaN6A 5B7
Ping-chin Cheng
Affiliation:
Advanced Microscopy and Imaging Laboratory, Dept. of Electrical Engineering, State University of New York, Buffalo, NY14260, USA
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The ability to visualize the chromosomes, especially in three-dimensional (3D) space, has great benefits for the understanding of genes’ function. The availability of various trisomic genotype in maize provides an excellent system for the study of 3D chromosome arrangement at various developmental stages. Laser scanning confocal microscopy and digital deconvolution technique is best suited for this type of work. A preliminary database on the three-dimensional arrangement of Ohio43 inbred line has been established; this information is essential for the evaluation of the effect of additional chromosome in trisomy genotypes.

Tassel inflorescence of maize (Zea mays L.) trisomy 6 in Ohio43 background were collected from the field station at the University of Western Ontario in London, Ontario, Canada, during the summer of 1998. Tassels at the proper developmental stages were fixed in 3:1 (95% EtOH / acetic acid) solution and stored at room temperature. After dissection from the spikelet, anthers were treated with the Feulgen-method, dehydrated in ethanol series and cleared in methyl salicylate.

Type
Ultrastructural Analysis Of Plant Cells
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1262 - 1263
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Cheng, P. C. et al., Maize Genetic Cooperation Newsletter, 73(1999).Google Scholar
2.Cheng, P. C. et al., Multidimensional Microscopy, Springer(1994).CrossRefGoogle Scholar
3.T. C., Lee et al., Maize Genetic Cooperation Newsletter, 73(1999).Google Scholar
4.Holmes, T. J. and Liu, Y. H., Visualization in Biomedical Microscopies, VCH(1992), 283–327.Google Scholar