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Three-Dimensional Structure of Drosophila Testis Tip: The Spatial Relation Between Dividing Cells

Published online by Cambridge University Press:  06 August 2013

Kyung Eun Lee ,
Sung Sik Han  and
Hyesung Jeon
Kyung Eun Lee*
Affiliation:
Advanced Analysis Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-g, Seoul 136-791, Republic of Korea
Sung Sik Han
Affiliation:
School of Life Sciences and Biotechnology, Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea
Hyesung Jeon
Affiliation:
Center for Theragnosis, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-g, Seoul 136-791, Republic of Korea
*
*Corresponding author. E-mail: kelee@kist.re.kr
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Abstract

At the apical tip of Drosophila testis, there is a stem cell niche known as the proliferation center, where the stem cells are maintained by hub cell cluster for the regulation of differentiation and proliferation. Germline stem cells go through mitosis four times from one primary spermatogonial cell to the 16-cell stage before the maturation. The cells derived from the same germline stem cell are located within one cyst, an enclosed system by two cyst cells, and they are connected by the intercellular bridges called ring canals. In this study, the three-dimensional (3D) structure of Drosophila testis tip was reconstructed from serial sections. The size of cells at each stage was compared in volume from the 3D structure. The stages of cells in a cyst could be distinguishable exactly by counting the cells linked with intercellular bridges in 3D-reconstructed structure. The cysts containing the same stage cells appeared in the horizontal plane. Both the germline stem cell directly attached to the hub cell and the spermatogonial cells detached from the hub cell were divided at the almost perpendicular direction to the spermatogonial cell layers. The dividing phase in one cyst was delayed gradually through the cytoplasmic region of intercellular bridge.

Keywords

3D reconstructionDrosophilatestisstem cell nichespermatogenesiscell division
Type
Research Article
Information
Microscopy and Microanalysis , Volume 19 , Supplement S5: IUMAS , August 2013 , pp. 188 - 193
Copyright
Copyright © Microscopy Society of America 2013 

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References

Beller, M., Riedel, D., Jansch, L., Dieterich, G., Wehland, J., Jackle, H. & Kuhnlein, R.P. (2006). Characterization of the Drosophila lipid droplet subproteome. Mol Cell Proteomics 5, 1082–1094.Google Scholar
de Cuevas, M. & Spradling, A.C. (1998). Morphogenesis of the Drosophila fusome and its implications for oocyte specification. Development 125, 2781–2789.10.1242/dev.125.15.2781Google Scholar
Deng, W. & Lin, H. (1997). Spectrosomes and fusomes anchor mitotic spindles during asymmetric germ cell divisions and facilitate the formation of a polarized microtubule array for oocyte specification in Drosophila. Dev Biol 189, 79–94.10.1006/dbio.1997.8669Google Scholar
Fuller, M.T. (1993). Spermatogenesis. In The Development of Drosophila melanogaster, Bate, M. & Arias, M. (Eds.), pp. 71–147. New York: Cold Spring Harber Laboratory Press.Google Scholar
Hardy, R.W., Tokuyasu, K.T., Lindsley, D.L. & Garavito, M. (1979). The germinal proliferation center in the testis of Drosophila melanogaster. J Ultrastruct Res 69, 180–190.10.1016/S0022-5320(79)90108-4Google Scholar
Hime, G.R., Brill, J.A. & Fuller, M.T. (1996). Assembly of ring canals in the male germ line from structural components of the contractile ring. J Cell Sci 109, 2779–2788.10.1242/jcs.109.12.2779Google Scholar
Kloc, M., Bilinski, S., Dougherty, M.T., Brey, E.M. & Etkin, L.D. (2004). Formation, architecture and polarity of female germline cyst in Xenopus. Dev Biol 266, 43–61.10.1016/j.ydbio.2003.10.002Google Scholar
Kremer, J.R., Mastronarde, D.N. & McIntosh, J.R. (1996). Computer visualization of three-dimensional image data using IMOD. J Struct Biol 116, 71–76.10.1006/jsbi.1996.0013Google Scholar
Lighthouse, D.V., Buszczak, M. & Spradling, A.C. (2008). New components of the Drosophila fusome suggest it plays novel roles in signaling and transport. Dev Biol 317, 59–71.Google Scholar
Lin, H. & Spradling, A.C. (1995). Fusome asymmetry and oocyte determination in Drosophila. Dev Genet 16, 6–12.Google Scholar
Lin, H., Yue, L. & Spradling, A.C. (1994). The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation. Development 120, 947–956.10.1242/dev.120.4.947Google Scholar
Robinson, D.N. & Cooley, L. (1996). Stable intercellular bridges in development: The cytoskeleton lining the tunnel. Trends Cell Biol 6, 474–479.10.1016/0962-8924(96)84945-2Google Scholar
Snapp, E.L., Iida, T., Frescas, D., Lippincott-Schwartz, J. & Lilly, M.A. (2004). The fusome mediates intercellular endoplasmic reticulum connectivity in Drosophila ovarian cysts. Mol Biol Cell 15, 4512–4521.10.1091/mbc.e04-06-0475Google Scholar
Spurr, A.R. (1969). A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26, 31–43.10.1016/S0022-5320(69)90033-1Google Scholar
Xie, T. & Spradling, A.C. (2000). A niche maintaining germ line stem cells in the Drosophila ovary. Science 290, 328–330.10.1126/science.290.5490.328Google Scholar
Yamashita, Y.M., Fuller, M.T. & Jones, D.L. (2005). Signaling in stem cell niches: Lessons from the Drosophila germline. J Cell Sci 118, 665–672.10.1242/jcs.01680Google Scholar