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Soft X-Ray Spectroscopy of Amino Acids and Peptides

Published online by Cambridge University Press:  02 July 2020

A. Osanna ,
J. Boese ,
C. Jacobsen  and
J. Kirz
A. Osanna
Affiliation:
Department of Physics, S.U.N.Y. at Stony Brook, Stony Brook, NY, 11794-3800
J. Boese
Affiliation:
now at Institute of Radiology and Pathophysiology, German Cancer Research Center, Heidelberg, Germany
C. Jacobsen
Affiliation:
Department of Physics, S.U.N.Y. at Stony Brook, Stony Brook, NY, 11794-3800
J. Kirz
Affiliation:
Department of Physics, S.U.N.Y. at Stony Brook, Stony Brook, NY, 11794-3800
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Extract

Soft x-rays provide a high signal-to-background ratio and an energy resolution of 0.1 - 0.5 eV for quantitative microanalysis of thin organic specimens. For specimens with non-uniform thickness, it it necessary to take spatially resolved spectra for quantitative analysis. In the Stony Brook scanning transmission x-ray microscope, spectra can be acquired from ∼ 0.2 micron spots, and images at selected energies can be acquired at < 55 nm Rayleigh resolution. This spectromicroscopy capability has been used for biological applications including the mapping of protein and DNA so as to rule out certain models of chromosome packing in sperm, the mapping of calcium in bone and tendon tissue, and for polymer science applications.

We have investigated x-ray absorption near edge structure (XANES) of amino acids and peptides at the carbon K-edge. We find strong evidence that spectra of small proteins can be predicted from amino acid spectra

Type
Quantitative Biological and Materials Microanalysis by Electrons and X-Rays
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 911 - 912
Copyright
Copyright © Microscopy Society of America 1997

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References

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