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Size-Controlled Synthesis of Cds Nanocrystals in Vesicles Confirmed in Situby Cryo-Transmission Electron Microscopy

Published online by Cambridge University Press:  02 July 2020

M. T. Kennedy ,
B. A. Korgel ,
J. A. Zasadzinski  and
H. G. Monbouquette
M. T. Kennedy
Affiliation:
Department of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA93106
B. A. Korgel
Affiliation:
Department of Chemical Engineering, University of California, Los Angeles, Los Angeles, CA90095
J. A. Zasadzinski
Affiliation:
Department of Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA93106
H. G. Monbouquette
Affiliation:
Department of Chemical Engineering, University of California, Los Angeles, Los Angeles, CA90095
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Extract

Size-quantized CdS nanocrystals, synthesized within phosphatidylcholine (PC) vesicles by reaction compartmentalization, were observed in situ to confirm the proposed method of formation and to investigate the concept of membrane-mediated crystal growth. Vesicle solutions representing three different CdS nanocrystal sizes, and a solution containing unreacted CdCl2 inside vesicles, were analyzed using cryo-transmission electron microscopy (cryo-TEM). Cryo-TEM has proved capable of resolving both the vesicle bilayer and the encapsulated nanocrystal, and yields previously unknown positional information for the crystal in relation to the bilayer.

Egg-PC vesicles containing Cd2+ were formed by detergent depletion, yielding vesicles of uniform size and a defined internal Cd2+ concentration. External Cd2+ was removed from the solution by cation exchange. Ammonium sulfide was added to the vesicle dispersion to produce nanocrystals via diffusion of S2- across the vesicle membrane. Particle size was predicted based upon the vesicle size, as determined from light scattering, and the encapsulated Cd2+ concentration.

Type
Nanocrystals and Nanocomposites: Novel Structures for Catalysis, Electronics, and Micromechanics
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 435 - 436
Copyright
Copyright © Microscopy Society of America 1997

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References

Korgel, B. A. and Monbouquette, H. G., J. Phys. Chem. 100 (1996) 346–351.10.1021/jp952138nCrossRefGoogle Scholar
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