Hostname: page-component-7bb8b95d7b-w7rtg Total loading time: 0 Render date: 2024-09-12T11:38:30.102Z Has data issue: false hasContentIssue false
  • English
  • Français

The Role Of P115 Rhogef In The Rho Mediated Effects Of Lpa On The Actin Cytoskeleton.

Published online by Cambridge University Press:  02 July 2020

C.L. Schwartz ,
C. Wells ,
X. Jiang ,
H.J. Arnott ,
P.C. Sternweis  and
M. A. Wilk-Blaszczak
C.L. Schwartz
Affiliation:
The Dept. of Biology, The University of Texas at Arlington, Arlington, TX76019 The Depts.of Anesthesiology and Pharmacology, Southwestern Medical Center at Dallas, Dallas, TX75235.
C. Wells
Affiliation:
The Depts.of Anesthesiology and Pharmacology, Southwestern Medical Center at Dallas, Dallas, TX75235.
X. Jiang
Affiliation:
The Depts.of Anesthesiology and Pharmacology, Southwestern Medical Center at Dallas, Dallas, TX75235.
H.J. Arnott
Affiliation:
The Dept. of Biology, The University of Texas at Arlington, Arlington, TX76019
P.C. Sternweis
Affiliation:
The Depts.of Anesthesiology and Pharmacology, Southwestern Medical Center at Dallas, Dallas, TX75235.
M. A. Wilk-Blaszczak
Affiliation:
The Depts.of Anesthesiology and Pharmacology, Southwestern Medical Center at Dallas, Dallas, TX75235.
Get access

Extract

In the fibroblast cell line, 3T3, lysophosphatidic acid (LPA) induces stress fiber formation. Stress fibers participate in physiological functions such as cell motility. LPA acts through a receptor coupled to a PTX-insensitive G-protein, G13. It was shown that a constitutively activated mutant of α13 (Q226L) induces stress fiber formation in Swiss3T3 cells through a second messenger cascade that involves a monomeric G-protein, Rho. The recently discovered guanine nucleotide exchange factor, p115 RhoGEF (p115) forms a link between a n and Rho A. In the presence of α13, p115 activates Rho. The N-terminus of p115 contains a regulator of G-protein signaling (RGS) box. RGS proteins act as negative regulators of G-protein dependent signaling by increasing GTPase activity and “locking” the G-protein in an inactive state. We have tested a role of p115 in the pathway coupling the LPA receptor to stress fiber formation by Rho in NTH-3T3 cells.

Type
Cytochemistry
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 1326 - 1327
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Ridley, A.J. and Hall, A. (1992) Cell 70, 389–399CrossRefGoogle Scholar
2.Gohla, A. et al. (1998) J. Biol. Chem 273,4653–4659CrossRefGoogle Scholar
3.Buhl, A.M. etal. (1995) J. Biol. Chem. 270, 24631–24634CrossRefGoogle Scholar
4.Hart, M.J. et al. (1998) Science 280,2112–2114CrossRefGoogle Scholar
5.Kozasa, T. et al. (1998) Science 280, 2109–2111CrossRefGoogle Scholar
6.Dohlman, H.G. and Thorner, J. (1997) J. Biol. Chem. 272, 3871–3874CrossRefGoogle Scholar