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Role of Nucleation-Promoting Factors in Mouse Early Embryo Development

Published online by Cambridge University Press:  04 April 2013

Qiao-Chu Wang ,
Jun Liu ,
Fei Wang ,
Xing Duan ,
Xiao-Xin Dai ,
Teng Wang ,
Hong-Lin Liu ,
Xiang-Shun Cui ,
Shao-Chen Sun  and
Nam-Hyung Kim
Qiao-Chu Wang
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Jun Liu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Fei Wang
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Xing Duan
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Xiao-Xin Dai
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Teng Wang
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Hong-Lin Liu
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Xiang-Shun Cui
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea
Shao-Chen Sun*
Affiliation:
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Nam-Hyung Kim*
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea
*
*Corresponding author. E-mail: sunsc@njau.edu.cn
**Corresponding author. E-mail: nhkim@chungbuk.ac.kr
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Abstract

During mitosis nucleation-promoting factors (NPFs) bind to the Arp2/3 complex and activate actin assembly. JMY and WAVE2 are two critical members of the NPFs. Previous studies have demonstrated that NPFs promote multiple processes such as cell migration and cytokinesis. However, the role of NPFs in development of mammalian embryos is still unknown. Results of the present study show that the NPFs JMY and WAVE2 are critical for cytokinesis during development of mouse embryos. Both JMY and WAVE2 are expressed in mouse embryos. After injection of JMY or WAVE2 siRNA, all embryos failed to develop to the morula or blastocyst stages. Moreover, using fluorescence intensity analysis, we found that the expression of actin decreased, and multiple nuclei were observed within a single cell indicating that NPFs-induced actin reduction caused the failure of cell division. In addition, injection of JMY and WAVE2 siRNA also caused ARP2 degradation, indicating that involvement of NPFs in development of mouse embryos is mainly through regulation of ARP2/3-induced actin assembly. Taken together, these data suggested that WAVE2 and JMY are involved in development of mouse embryos, and their regulation may be through a NPFs-Arp2/3-actin pathway.

Keywords

JMYWAVE2ARP2/3actinembryo development
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 3 , June 2013 , pp. 559 - 564
Copyright
Copyright © Microscopy Society of America 2013 

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