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A Review of Ultrastructural and Ultracytochemical Studies of Infection Processes in Some Plant Wilt Diseases: The Opaque Matter Extensively Involved, Its Links with Pathogen Elements, Insights into Its Nature

Published online by Cambridge University Press:  03 February 2011

G.B. Ouellette ,
P.M. Charest  and
H. Chamberland
G.B. Ouellette*
Affiliation:
3413, rue de Sarnia, Quebec (QC) G1X 2K5, Canada
P.M. Charest
Affiliation:
Département de Phytologie Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec (QC), Canada
H. Chamberland
Affiliation:
Pavillon C.E. Marchand, Université Laval, Québec (QC), Canada
*
Corresponding author. E-mail: gouellette_3@sympatico.ca
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Abstract

The present review is based on results of several years of investigation on the pathogenesis of plant wilt diseases using for one of the main aspects, several labeling techniques at the ultrastructural level: autoradiography with 3H-thymidine; gold-tagged lectins, enzymes, polyclonal, and monoclonal antibodies. Thus, opaque matter (OM), labeling for DNA with the appropriate markers, can be abundant in and associated with host tissue reactions and pronounced alterations (particularly in newly differentiated xylem). The presence of structures (called P-elements) in OM, paralleled by a similar occurrence in nuclei of both the host and pathogen cells, is considered as being also indicative of a cytoplasmic nature for this OM and most likely originating from fungal elements.

Keywords

DNA gold labelingcellulosechitinfungal extracellular material
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 2 , April 2011 , pp. 137 - 155
Copyright
Copyright © Microscopy Society of America 2011

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References

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