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Neural Circuit Reconstruction Using Serial Block-face Scanning Microscopy (SBFSEM) in Mouse Retina

Published online by Cambridge University Press:  30 July 2020

Wan-Qing Yu ,
Chi-Chou Huang ,
Hideki Sasaki ,
Sean McElroy ,
Gaoyu Xiao ,
James Lee ,
Luciano Lucas  and
Rachel Wong
Wan-Qing Yu
Affiliation:
University of Washington, Seattle, Washington, United States
Chi-Chou Huang
Affiliation:
DRVision Technologies LLC, Bellevue, Washington, United States
Hideki Sasaki
Affiliation:
DRVision Technologies LLC, Bellevue, Washington, United States
Sean McElroy
Affiliation:
DRVision Technologies LLC, Bellevue, Washington, United States
Gaoyu Xiao
Affiliation:
DRVision Technologies LLC, Bellevue, Washington, United States
James Lee
Affiliation:
DRVision Technologies LLC, Bellevue, Washington, United States
Luciano Lucas
Affiliation:
DRVision Technologies LLC, Bellevue, Washington, United States
Rachel Wong
Affiliation:
University of Washington, Seattle, Washington, United States

Abstract

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Type
3D Scanning Electron Microscopy Imaging of Biological Samples
Information
Microscopy and Microanalysis , Volume 26 , Supplement S2 , August 2020 , pp. 590
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Copyright
Copyright © Microscopy Society of America 2020

References

Briggman, K. L., & Bock, D. D. (2012). Volume electron microscopy for neuronal circuit reconstruction. Current opinion in neurobiology, 22(1), 154-161.10.1016/j.conb.2011.10.022CrossRefGoogle ScholarPubMed
Denk, W, Horstmann, H. Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol 2004, 2:e329.10.1371/journal.pbio.0020329CrossRefGoogle ScholarPubMed
Bishop, D, Nikic, I, Brinkoetter, M, Knecht, S, Potz, S, Kerschensteiner, M, Misgeld, T. Near-infrared branding efficiently correlates light and electron microscopy. Nat Methods 2011, 8:568-570.10.1038/nmeth.1622CrossRefGoogle ScholarPubMed