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The Nature and Distribution of Large Proteoglycans in Developing Rat Bone.

Published online by Cambridge University Press:  02 July 2020

I. Lee
Affiliation:
Department of Anatomy and Chiyoda-ku, Tokyo101, Japan
Y. Ono
Affiliation:
Department of Anatomy and Chiyoda-ku, Tokyo101, Japan
N. Kubushiro
Affiliation:
Department of Anatomy and Chiyoda-ku, Tokyo101, Japan
T. Ikeda
Affiliation:
Dental Technician Training School, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo101, Japan
M. Takagi
Affiliation:
Department of Anatomy and Chiyoda-ku, Tokyo101, Japan
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Extract

The nature and distribution of large hyaluronate-binding proteoglycans (PGs) in early bone formation bf developing rat mandible were biochemically and immunohistochemically investigated with a monoclonal antibody (MAb) 5D5, which is derived from bovine sciera and specifically recognizes the core protein of the large PGs such as versican, neurocan and brevican, but not that of aggrecan.

The first sign of mandibular bone formation is evident in the embryonic day 15 (Day 15). At the site of initiation of bone formation, a center of ossification was seen in the close proximity to Meckel’s cartilage. At the light microscopic level, these centers contained fine strips of the intercellular matrix surrounded by osteoblasts (Fig. 1). At the EM level, these matrices contained very fine fibrillar material, numerous, osteoblastic cell processes, and vesicular structures, but did not show any typical mineralization pattern. In Day 16, clusters of needle-like crystals or bone nodules, which varied considerably in size and shape, were seen in the intercellular matrix among osteoblasts (Fig. 2).

Type
Developmental and Reproductive Biology
Copyright
Copyright © Microscopy Society of America 1997

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References

1.Rahemtulla, F.et al., J. Dent. Res., 373(1988)2086Google Scholar
2.Bratt, P.et al., Int. J. Biochem., 24(1992)157310.1016/0020-711X(92)90173-XCrossRefGoogle Scholar
3.Bernald, G.W.and Pease, D.C., Am. J. Anat., 125(1969)27110.1002/aja.1001250303CrossRefGoogle Scholar