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Investigating gating mechanisms of ion channels using temperature-resolved cryoEM

Published online by Cambridge University Press:  30 July 2021

Harsh Bansia ,
Claudio Catalano ,
Zephan Melville ,
Youzhong Guo ,
Andrew R. Marks  and
Amedee des Georges
Harsh Bansia
Affiliation:
CUNY Advanced Science Research Center, New York, United States
Claudio Catalano
Affiliation:
Virginia Commonwealth University, Richmond, Virginia, United States
Zephan Melville
Affiliation:
Columbia University, New York, United States
Youzhong Guo
Affiliation:
Virginia Commonwealth University, Richmond, Virginia, United States
Andrew R. Marks
Affiliation:
Columbia University, New York, United States
Amedee des Georges
Affiliation:
CUNY Advanced Science Research Center, New York, United States

Abstract

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Type
3D Structures: From Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 27 , Supplement S1 , August 2021 , pp. 1690 - 1694
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

References

Cheng, Y. Membrane protein structural biology in the era of single particle cryo-EM. Curr Opin Struct Biol, 52, 2018.Google ScholarPubMed
Qiu, W, et al. Structure and activity of lipid bilayer within a membrane-protein transporter. Proc Natl Acad Sci U S A, 115, 2018.Google ScholarPubMed
Chowdhury, S, et al. A molecular framework for temperature-dependent gating of ion channels. Cell, 158, 2014.CrossRefGoogle ScholarPubMed
Moskvin, AS, et al. The temperature effect on cardiac ryanodine receptor gating and conductance: Mathematical modeling. BIOPHYSICS 61, 2016.Google Scholar
Koprowski, P, et al. Negative and positive temperature dependence of potassium leak in MscS mutants: Implications for understanding thermosensitive channels. Biochim Biophys Acta, 1848, 2015.Google Scholar
Mehra, R, et al. Cryo-temperature effects on membrane protein structure and dynamics. Phys Chem Chem Phys, 22, 2020.Google ScholarPubMed
Santulli, G, et al. Physiology and pathophysiology of excitation-contraction coupling: the functional role of ryanodine receptor. J Muscle Res Cell Motil, 38, 2017.CrossRefGoogle ScholarPubMed
Ogawa, H, et al. Regulatory mechanisms of ryanodine receptor/Ca2+ release channel revealed by recent advancements in structural studies. J Muscle Res Cell Motil, 2020.Google Scholar
des Georges, A, et al. Structural Basis for Gating and Activation of RyR1. Cell, 167, 2016.Google ScholarPubMed
Naismith, JH, Booth, IR. Bacterial mechanosensitive channels-MscS: evolution's solution to creating sensitivity in function. Annu Rev Biophys, 41, 2012.CrossRefGoogle Scholar
Chen, CY, et al. Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions. J Am Chem Soc, 141, 2019.CrossRefGoogle ScholarPubMed
Tegunov, D & Cramer, P. Real-time cryo-electron microscopy data preprocessing with Warp. Nat Methods, 16, 2019.Google ScholarPubMed
Punjani, A, et al. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat Methods, 14, 2017.CrossRefGoogle ScholarPubMed
Frank, J & Ourmazd, A. Continuous changes in structure mapped by manifold embedding of single-particle data in cryo-EM. Methods, 100, 2016.CrossRefGoogle ScholarPubMed
Dashti, A, et al. Retrieving functional pathways of biomolecules from single-particle snapshots. Nat Commun, 11, 2020.CrossRefGoogle ScholarPubMed