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Industrial and Clinical Applications of Infrared Spectroscopic Imaging Using Focal-Plane Arrays

Published online by Cambridge University Press:  02 July 2020

Linda H. Kidder ,
Ira W. Levin  and
E. Neil Lewis
Linda H. Kidder
Affiliation:
Laboratory of Chemical Physics, National Institute of Diabetes, and Digestive and Kidney Diseases, The National Institutes of Health, 9000 Rockville Pike, Building 5, Room B 1-38, Bethesda, MD, 20892
Ira W. Levin
Affiliation:
Laboratory of Chemical Physics, National Institute of Diabetes, and Digestive and Kidney Diseases, The National Institutes of Health, 9000 Rockville Pike, Building 5, Room B 1-38, Bethesda, MD, 20892
E. Neil Lewis
Affiliation:
Laboratory of Chemical Physics, National Institute of Diabetes, and Digestive and Kidney Diseases, The National Institutes of Health, 9000 Rockville Pike, Building 5, Room B 1-38, Bethesda, MD, 20892
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Extract

Conventional Fourier transform infrared (FTIR) spectroscopy is a workhorse analytical technique providing quantitative and qualitative information on a vast array of materials, ranging from semiconductors to biological samples. By coupling a Michelson interferometer and infrared (IR) microscope to an IR sensitive focal-plane array detector, we have added an imaging component to the existing analytical capabilities of FTIR spectroscopy. This additional capability makes it possible to image the distribution of subtle structural and compositional changes reflected in the infrared spectrum within heterogeneous materials. As such, it is an extremely powerful analytical tool which is currently employed in a variety of basic research and industrial applications. Similarly the utility of FTIR spectroscopy for the investigation of biological materials can be greatly extended by recording spectroscopic imaging data sets instead of single FTIR spectra. This spectroscopic imaging capability, especially when combined with microscopy, ensures that information concerning spatial variations in the system’s biochemistry, as well as its cellular morphology, is maintained.

Type
Compositional Mapping With High Spatial Resolution
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 156 - 157
Copyright
Copyright © Microscopy Society of America

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References

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