Hostname: page-component-7479d7b7d-qlrfm Total loading time: 0 Render date: 2024-07-11T12:25:17.105Z Has data issue: false hasContentIssue false

In Situ Structural Analysis of Bacterial Nanomachines by Combining Cryo-FIB Milling, Cryo-ET and Sub-tomogram Analysis

Published online by Cambridge University Press:  30 July 2020

Yunjie Chang
Affiliation:
Department of Microbial Pathogenesis, Yale University School of Medicine, West Haven, Connecticut, United States Microbial Sciences Institute, Yale University, West Haven, Connecticut, United States
Jun Liu
Affiliation:
Department of Microbial Pathogenesis, Yale University School of Medicine, West Haven, Connecticut, United States Microbial Sciences Institute, Yale University, West Haven, Connecticut, United States

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
The Promise of Cryo-Electron Tomography
Copyright
Copyright © Microscopy Society of America 2020

References

Mastronarde, D.N., Automated electron microscope tomography using robust prediction of specimen movements. Journal of Structural Biology, 2005. 152(1): p. 36-51.10.1016/j.jsb.2005.07.007CrossRefGoogle ScholarPubMed
Zheng, S.Q., et al. ., MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nature Methods, 2017. 14: p. 331.10.1038/nmeth.4193CrossRefGoogle ScholarPubMed
Kremer, J.R., Mastronarde, D.N., and McIntosh, J.R., Computer Visualization of Three-Dimensional Image Data Using IMOD. Journal of Structural Biology, 1996. 116(1): p. 71-76.10.1006/jsbi.1996.0013CrossRefGoogle ScholarPubMed
Zhang, K., Gctf: Real-time CTF determination and correction. J Struct Biol, 2016. 193(1): p. 1-12.10.1016/j.jsb.2015.11.003CrossRefGoogle ScholarPubMed
Winkler, H., 3D reconstruction and processing of volumetric data in cryo-electron tomography. Journal of Structural Biology, 2007. 157(1): p. 126-137.10.1016/j.jsb.2006.07.014CrossRefGoogle ScholarPubMed
Winkler, H., et al. ., Tomographic subvolume alignment and subvolume classification applied to myosin V and SIV envelope spikes. Journal of Structural Biology, 2009. 165(2): p. 64-77.10.1016/j.jsb.2008.10.004CrossRefGoogle ScholarPubMed
Chang, Y., et al. ., Structural insights into flagellar stator-rotor interactions. Elife, 2019. 8.10.7554/eLife.48979CrossRefGoogle ScholarPubMed