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Immunocytochemical Analysis of Murine Peritoneal Macrophages Treated with “Poly-Plat”, an in Vitro and in Vivo Study

Published online by Cambridge University Press:  02 July 2020

H. J. Muenchen  and
S.K. Aggarwal
S.K. Aggarwal
Affiliation:
Department of Zoology, Michigan State University, East Lansing, MI48824-1115
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Extract

Poly-[(trans-1,2-diaminocyclohexane) platinumj-carboxyamylose (“poly-plat“) is a second generation analog of cisplatin which enhances the immune system with greater efficacy in vitro and in vivo “Poly-plat” contains 1/5 the platinum of CDDP and demonstrates less toxicity. In order to understand the mechanism of action of this compound an in vitro and in vivo study was performed. Swiss Webster mice and isolated murine peritoneal macrophages were treated with “poly-plat” (10 mg/kg). The Swiss Webster mice were given bolus injections and sacrificed at 2 and 12 days. Peritoneal macrophages were then isolated and allowed to incubate in culture for 24 h. Peritoneal macrophages were also isolated from normal mice and treated with the drugs for 2 h. After treatments the macrophages were placed in fresh media and allowed to incubate 24 h. Supematants were isolated at various times during culture for immunocytochemical analysis.

Both in vitro and in vivo studies showed enhanced immunostimulation after their respective treatments.

Type
Cytochemistry (Light and Electron Histochemistry)
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1122 - 1123
Copyright
Copyright © Microscopy Society of America

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References

1. Muenchen, H.J. and Aggarwal, S.K., Immune system activation by cisplatin and its analog “poly-plat“: an in vitro and in vivo study. Anti-cancer Drugs 9(1998)In Press.Google Scholar

2. Fiebig, H.H. et al., GB-21, a novel platinum polymer complex with antitumor activity in human renal and mammary xenografts. Proc. Amer. Cancer Res. 37(1996)297.Google Scholar

3. Palma, J.P. and Aggarwal, S.K., Cisplatin and carboplatin mediated release of cytolytic factors in murine peritoneal macrophages in vitro. Anti-cancer Drugs 3(1992)665.CrossRefGoogle Scholar