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Imaging and Reconstructing Microglia in 3 Dimensions Using FIB-SEM

Published online by Cambridge University Press:  05 August 2019

Julie C Savage ,
Sammy Weiser Novak  and
Marie-Eve Tremblay
Julie C Savage
Affiliation:
Axe neurosciences, Centre de Recherche du CHU de Québec-Université Laval, Québec City, QC, Canada.
Sammy Weiser Novak
Affiliation:
Axe neurosciences, Centre de Recherche du CHU de Québec-Université Laval, Québec City, QC, Canada. Salk Institute for Biological Studies, La Jolla, CA, USA.
Marie-Eve Tremblay*
Affiliation:
Axe neurosciences, Centre de Recherche du CHU de Québec-Université Laval, Québec City, QC, Canada. Départment de Médecine Moleculaire, Université Laval, Quebec City, QC, Canada.
*
*Corresponding author: tremblay.marie-eve@crchudequebec.ulaval.ca

Abstract

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Type
3D Structures: from Macromolecular Assemblies to Whole Cells (3DEM FIG)
Information
Microscopy and Microanalysis , Volume 25 , Supplement S2 , August 2019 , pp. 1316 - 1317
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Ransohoff, RM and El Khoury, J, Cold Spring Harbor Perspectives in Biology. 8 (2015), p. a020560.Google Scholar
[2]Tay, TL et al. , J. Physiol. (Lond.). 595 (2017), p. 1929.Google Scholar
[3]Savage, JC et al. , Front Immunol. 9 (2018), p. 803.Google Scholar
[4]Tremblay, M-È, Lowery, RL and Majewska, AK, PLoS Biol. 8 (2010), p. e1000527.Google Scholar
[5]Maco, B et al. , Nature Protocols. 9 (2014), p. 1354.Google Scholar
[6]Tremblay, M-È, Riad, M and Majewska, A, J Vis Exp (2010), doi:10.3791/2021.Google Scholar
[7]JCS holds funding from an FRQS postdoctoral fellowship, and MET is funded by ERANET MicroSynDep, CIHR Foundation Scheme, and NSERC Discovery grants.Google Scholar