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Glutathione Distribution in Transformed Leukocytes Determined by Immunoelectron Microscopy

Published online by Cambridge University Press:  02 July 2020

Jeffrey G. Ault  and
David A. Lawrence
Jeffrey G. Ault
Affiliation:
Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York, 12201-0509, USA
David A. Lawrence
Affiliation:
Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Albany, New York, 12201-0509, USA
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Abstract

Glutathione (GSH) is a tripeptide thiol found in virtually all mammalian cells. GSH is a cofactor in several enzymatic reactions and is important in intracellular cysteine storage. Probably the most interesting of its many functions is its role in protecting the cell against the detrimental effects of ionizing radiation, reactive oxygen compounds, free radicals, and toxic xenobiotics. GSH is the reduced form which can be oxidized to a disulfide form (GSSG) or to form mixed disulfides with proteins. The GSH oxidation-reduction cycle maintains a high GSH to GSSG ratio which provides a reservoir of reducing equivalents that can nullify reactive oxygen species and detoxify xenobiotics. The study of glutathione in the fields of cancer research and immunology has attracted much attention, especially with regard to changes with age.

In this study, we have determined the cellular distribution of glutathione using immunoelectron microscopy. A mouse monoclonal antibody (8.1-GSH) against the glutathione adduct with Nefhylmaleimide (NEM) was used.

Type
Biological Ultrastructure (Cells, Tissues, Organ Systems)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 70 - 71
Copyright
Copyright © Microscopy Society of America 2001

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References

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