Hostname: page-component-76fb5796d-qxdb6 Total loading time: 0 Render date: 2024-04-26T06:52:57.611Z Has data issue: false hasContentIssue false

Exchange Dynamics of Dynamin Measured in Living Cells During Endocytic Vesicle Formation

Published online by Cambridge University Press:  05 August 2019

Léa Claverie
Affiliation:
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
Morgane Rosendale
Affiliation:
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
Patrice Maschalchi
Affiliation:
Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, University of Bordeaux, Bordeaux, France.
Corey Butler
Affiliation:
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
Natacha Retailleau
Affiliation:
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
Jean-Baptiste Sibarita
Affiliation:
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
Daniel Choquet
Affiliation:
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France. Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, University of Bordeaux, Bordeaux, France.
David Perrais*
Affiliation:
University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France CNRS, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 Bordeaux, France.
*
*Corresponding author: david.perrais@u-bordeaux.fr

Abstract

Image of the first page of this content. For PDF version, please use the ‘Save PDF’ preceeding this image.'
Type
Light and Fluorescence Microscopy for Imaging Cell Surface and Cell Structure
Copyright
Copyright © Microscopy Society of America 2019 

References

[1]Dar, S, Kamerkar, SC, Pucadyil, TJ. A high-throughput platform for real-time analysis of membrane fission reactions reveals dynamin function. Nat Cell Biol 17:15881596 (2015)Google Scholar
[2]Kong, L, Sochacki, KA, Wang, H, Fang, S, Canagarajah, B, Kehr, AD, et al. Cryo-EM of the dynamin polymer assembled on lipid membrane. Nature 560:258-262 (2018)Google Scholar
[3]Shupliakov, O, Löw, P, Grabs, D, Gad, H, Chen, H, David, C, et al. Synaptic vesicle endocytosis impaired by disruption of dynamin-SH3 domain interactions. Science 276:259263 (1997)Google Scholar
[4]Merrifield, CJ, Perrais, D, Zenisek, D. Coupling between Clathrin-Coated-Pit Invagination, Cortactin Recruitment, and Membrane Scission Observed in Live Cells. Cell 121:593606 (2005)Google Scholar
[5]Taylor, MJ, Perrais, D, Merrifield, CJ. A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis. Schmid SL, editor. PLoS Biol 9:e1000604 (2011)Google Scholar
[6]The authors acknowledge funding from the Centre National de la Recherche Scientifique, the Agence Nationale de la Recherche (ANR-12-BSV5-0005 “CaPeBlE”) and the Aquitaine Regional Council.Google Scholar