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Electrostatic Capture Following Laser Microdissection for the Preparation of Homogeneous Biological Specimens

Published online by Cambridge University Press:  28 November 2016

Dingrong Yi ,
Linghua Kong ,
Ranjith K. Kankala  and
Zi Wang
Dingrong Yi*
Affiliation:
College of Mechanical Engineering and Automation, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
Linghua Kong
Affiliation:
College of Mechanical Engineering and Automation, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
Ranjith K. Kankala
Affiliation:
College of Chemical Engineering, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
Zi Wang
Affiliation:
College of Mechanical Engineering and Automation, Huaqiao University, No. 668 Jimei Road, Jimei District, Xiamen, Fujian Province 361021, P. R. China
*
*Corresponding author. yidr@hqu.edu.cn
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Abstract

There is an unmet need for researchers in life sciences and clinical pathology to obtain untainted target cells with very high accuracy, which are suitable for subsequent genome and protein analysis. In this paper, an electrostatic capture laser microdissection technology (ECM) is proposed and explained. Following microscopic identification and separation of target cells from the surrounding tissues using laser cutting, the ECM uses electrostatic forces to capture target cells. Validation experiments indicate that ECM can capture a wide assortment of contamination-free homogeneous samples, ranging from very tiny pieces of a few micrometers in diameter to large pieces with a surface area of over 40,000 µm2. Evidence is also provided indicating that uncontaminated homogeneous tissue materials collected by ECM are suitable for further DNA and RNA analysis. This suggests that ECM capture causes little or no identifiable damage to the collected tissues. This technique has significant advantages compared with existing traditional capture methods, such as very low risk of biological sample damage and the fact that it can be applied to both upright and inverted microscopy. The latter allows for separating target cells in tissue culture. ECM usage provides a cost-effective alternative to more traditional laser capture microdissection techniques.

Keywords

electrostatic captureelectrostatic forcecontact-freecontamination-freelaser micro-dissection
Type
Instrumentation and Software Techniques
Information
Microscopy and Microanalysis , Volume 22 , Issue 6 , December 2016 , pp. 1329 - 1337
Copyright
© Microscopy Society of America 2016 

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