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Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface

Published online by Cambridge University Press:  23 February 2017

Odair Bim Júnior ,
Marco A. Cebim ,
Maria T. Atta ,
Camila M. Machado ,
Luciana F. Francisconi-dos-Rios  and
Linda Wang
Odair Bim Júnior
Affiliation:
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, 17012-901 SP, Brazil
Marco A. Cebim
Affiliation:
Department of Inorganic Chemistry, Institute of Chemistry, Universidade Estadual Paulista, Araraquara, 14800-060 SP, Brazil
Maria T. Atta
Affiliation:
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, 17012-901 SP, Brazil
Camila M. Machado
Affiliation:
Department of Prosthodontics, Bauru School of Dentistry, University of São Paulo, Bauru, 17012-901 SP, Brazil
Luciana F. Francisconi-dos-Rios
Affiliation:
Department of Operative Dentistry, School of Dentistry, University of São Paulo, São Paulo, 05508-000 SP, Brazil
Linda Wang*
Affiliation:
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, 17012-901 SP, Brazil
*
*Corresponding author. wang.linda@usp.br
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Abstract

Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to determine suitable concentrations of RB in these bonding agents for imaging in the confocal laser scanning microscope. This study provides systematical strategies for adding RB to viscous dental adhesive resins, focusing on the determination of the lowest range of dye concentrations necessary to achieve an acceptable image of the dentin/adhesive interface. It was demonstrated that optimized images of the resin distribution in dentin can be produced with 0.1–0.02 mg/mL of RB in the (tested) adhesives. Our approaches took into account aspects related to the dye concentration, photophysical parameters in different host media, specimen composition and morphology to develop a rational use of the fluorescent agent with the resin-based materials. Information gained from this work can help optimize labeling methods using dispersions of low-molecular-weight dyes in different monomer blend systems.

Keywords

confocal laser microscopydental adhesivesdentin bondingfluorescent dyesphotoluminescence spectroscopyinterfacial morphology
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 23 , Issue 1 , February 2017 , pp. 122 - 130
Copyright
© Microscopy Society of America 2017 

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