Hostname: page-component-77c89778f8-rkxrd Total loading time: 0 Render date: 2024-07-25T05:10:04.775Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of Early Cytomegalovirus Infection in Pathologial Specimens by H&E, IHC, ISH, Grocott’S and Modified Steiner Silver Stain for LM and EM: A Review

Published online by Cambridge University Press:  02 July 2020

K. Chien ,
ML. Heathershaw ,
R.C. Heusser ,
H. Shiroishi ,
R. Gonzalez ,
C.C. Nast  and
A.H. Cohen
K. Chien
Affiliation:
Dept. of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, 90048
ML. Heathershaw
Affiliation:
Dept. of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, 90048
R.C. Heusser
Affiliation:
Dept. of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, 90048
H. Shiroishi
Affiliation:
Dept. of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, 90048
R. Gonzalez
Affiliation:
Dept. of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, 90048
C.C. Nast
Affiliation:
Dept. of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, 90048
A.H. Cohen
Affiliation:
Dept. of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, 90048
Get access

Abstract

The diagnosis of cytomegalovirus (CMV) in tissue sections is usually quite easy because of the characteristic appearance of intranuclear inclusions. At times the morphological features are equivocal, the nuclear findings may be atypical or only a single cell may be affected, thereby creating uncertainty about the diagnosis. This report reviews our experience with the various microscopic techniques that can be used to make definitive identification of CMV.

EM - Visualizing the viral particles in a single or even lysed cell by routine electron microscopy can make the definite diagnosis, but section size limits the usefulness in the detection of early CMV infection (fig. 1). However, if a diagnosis can not be made with certainty by the following methods, EM can be performed on stained paraffin sections for confirmation.

H&E - A Hematoxylin & Eosin stain that discloses characteristic intranuclear inclusions surrounded by a clear halo indicates CMV infection (fig.2A).

Type
Emerging Pathogens: Something Old, Something New (Organized By S. Miller and D. Howell)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 178 - 179
Copyright
Copyright © Microscopy Society of America 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

references

1.Wu, G.D.et al., Amer. J. Gastroenterology, 84(1989)1517.Google Scholar
2.Chien, K.et al., Proc. Ann EMSA Meeting 47(1989)1046.Google Scholar
3.Chien, K.et al., International Congress for E. M. 12(1990)808.Google Scholar
4.Chien, K.et al., Proc. Ann EMSA Meeting 49(1991)82.Google Scholar