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CTF Corrected Structure of the Closed-State Calcium Release Channel

Published online by Cambridge University Press:  02 July 2020

S. Ludtke ,
I. Serysheva ,
S. L. Hamilton  and
W. Chiu
S. Ludtke
Affiliation:
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX77030
I. Serysheva
Affiliation:
Dept. of Molecular Physiology and Biophysics, Baylor College of Medicine, TX77030
S. L. Hamilton
Affiliation:
Dept. of Molecular Physiology and Biophysics, Baylor College of Medicine, TX77030
W. Chiu
Affiliation:
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX77030
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Extract

Several structures of the Ryanodine receptor in different functional states with various bound ligands have been determined using electron cryo-microscopy in recent years. These structures have varied in resolution from 25-40Å, however, to date, none have been corrected for the CTF (contrast transfer function) of the microscope. The CTF can have a significant effect on the detailed structure of the molecule, causing features in the 40-70Å range to be unnaturally emphasized. This causes small holes to become larger and small protrusions to become elongated. Unfortunately due to zero points in the CTF, correction cannot be properly accomplished with individual images. Several approaches to correcting the CTF have been proposed and/or used by combining information from images taken at different defocus settings. We have taken the approach of performing CTF correction as a preprocessing step. There are both advantages and disadvantages to this method. There are 2 primary advantages: First, by combining data from identical regions of focal pairs,

Type
Imaging of Macromolecular Complexes
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 950 - 951
Copyright
Copyright © Microscopy Society of America

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References

References:

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5. This research was supported by NIH grants RR02250, AR41729.Google Scholar