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Cryo-FIB Sample Preparation for Cryo-ET With the Volta Phase Plate

Published online by Cambridge University Press:  25 July 2016

Miroslava Schaffer ,
Benjamin D. Engel ,
Marc Wehmer ,
Sahradha Albert ,
Eri Sakata ,
Anna Rast ,
Jörg Nickelsen ,
Jürgen M. Plitzko  and
Wolfgang Baumeister
Miroslava Schaffer
Affiliation:
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, Martinsried, Germany
Benjamin D. Engel
Affiliation:
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, Martinsried, Germany
Marc Wehmer
Affiliation:
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, Martinsried, Germany
Sahradha Albert
Affiliation:
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, Martinsried, Germany
Eri Sakata
Affiliation:
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, Martinsried, Germany
Anna Rast
Affiliation:
Biozentrum Ludwig-Maximilians-Universität (LMU), Department Biologie I - Botanik, Grosshaderner Strasse 2-4, Martinsried, Germany
Jörg Nickelsen
Affiliation:
Biozentrum Ludwig-Maximilians-Universität (LMU), Department Biologie I - Botanik, Grosshaderner Strasse 2-4, Martinsried, Germany
Jürgen M. Plitzko
Affiliation:
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, Martinsried, Germany
Wolfgang Baumeister
Affiliation:
Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, Martinsried, Germany

Abstract

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Abstract
Information
Microscopy and Microanalysis , Volume 22 , Supplement S3: Proceedings of Microscopy & Microanalysis 2016 , July 2016 , pp. 72 - 73
Copyright
© Microscopy Society of America 2016 

References

References:

[1] Rigort, A, et al, PNAS 109(12 (2012). p. 4449.Google Scholar
[2] Schaffer, M, et al, Bio-protocol 5(17 (2015) e1575.CrossRefGoogle Scholar
[3] Engel, B, et al, eLife (2015) 4, e04889.CrossRefGoogle Scholar
[4] Engel, B, et al, PNAS 112(36 (2015). p. 11264.CrossRefGoogle Scholar
[5] Danev, R, et al, PNAS 111(44 (2014). p. 15635.CrossRefGoogle Scholar
[6] Schaffer, M, et al, Microsc. Microanal 21(Suppl 3 (2015). p. 1119.CrossRefGoogle Scholar
[7] Mahamid, J, et al, Science, 2016, accepted.Google Scholar
[8] Asano, S, et al, J Mol Biol 428 (2016). p. 332.CrossRefGoogle Scholar
[9] This work was supported by the European Commission grant agreement ERC-2012-SyG_318987-ToPAG, the Deutsche Forschungsgemeinschaft Excellence Clusters CIPSM and SFB 1035, and the Max Planck Society.Google Scholar