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Cryo-Electron Microscopy of Spiroplasma Virus SpV4

Published online by Cambridge University Press:  02 July 2020

P.R. Chipman ,
R. Mckenna ,
J. Renaudin  and
T.S. Baker
P.R. Chipman
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, Indiana47907
R. Mckenna
Affiliation:
Department of Biological Sciences, University of Warwick, CoventryCV8 7AL; UK.
J. Renaudin
Affiliation:
Laboratoire de Biologie Cellulaire et Molé culaire, INRA et Université de Bordeaux II, 33883Villenave d’Ornon Cedex, France
T.S. Baker
Affiliation:
Department of Biological Sciences, Purdue University, West Lafayette, Indiana47907
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Extract

Spiroplasma, a wall-free prokaryote of the class Mollicutes, is host to a small, naked, single-stranded DNA, isometric virus. Spiroplasma virus SpV4 belongs to the Microviridae family, members of which are non-enveloped, have icosahedral capsids, release progeny through a lytic cycle, and contain circular DNA.

Measurements obtained from negatively stained SpV4 particles revealed a nucleocapsid of 27nm in diameter (figure 1). The three-dimensional structure reported here, obtained from unstained particles suspended in a layer of vitreous ice (figure 2), is in agreement with these earlier results, suggesting a 27nm average distance through the nucleocapsid (figure 3). Unreported in earlier studies is the presence of a 6nm, mushroom-shaped protrusion (made up of a stalk, 2.3nm long and 1.3nm wide, and a globular bud of dimensions ≈4.0×4.0×3.7nm) stemming from an ≈1.5nm deep depression at each of the 3-fold icosahedral axes of the virion. A cross section through the longitudinal axis of one protuberance (figure 4) reveals a cylindrical dimple (≈1.0nm in diameter and 2.3nm deep), originating on the axis of the outer surface of the globular bud domain.

Type
Solving Microbiological Problems With Microscopy
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 87 - 88
Copyright
Copyright © Microscopy Society of America 1997

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