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Correlative Light and Electron Microscopy (CLEM) on Biological Samples Using Immuno Electron Microscopy

Published online by Cambridge University Press:  23 September 2015

C. ten Brink ,
V. Oorschot  and
J. Klumperman
C. ten Brink
Affiliation:
Dept of Cell Biology, Cell Microscopy Core, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, The Netherlands
V. Oorschot
Affiliation:
Dept of Cell Biology, Cell Microscopy Core, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, The Netherlands Current address: Monash University, Melbourne, Australia
J. Klumperman
Affiliation:
Dept of Cell Biology, Cell Microscopy Core, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, The Netherlands

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 21 , Supplement S3: Proceedings of Microscopy & Microanalysis 2015 , August 2015 , pp. 1379 - 1380
Copyright
Copyright © Microscopy Society of America 2015 

References

References:

1 Slot, J.W. & Geuze, H.J., Cryosectioning and immunolabeling. Nature protocols, 2007. 2(10): p. 2480–2491.CrossRefGoogle ScholarPubMed
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3 van Rijnsoever, C., Oorschot, V. & Klumperman, J. ,Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections. Nat Methods, 2008. 5(11): p. 973–980.CrossRefGoogle ScholarPubMed
4 Cortese, K., et al., High data output method for 3-D correlative light-electron microscopy using ultrathin cryosections. Methods Mol Biol (2013) 950, p. 417–437.CrossRefGoogle ScholarPubMed