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Bacterial Attachment to Oral Epithelium

Published online by Cambridge University Press:  02 July 2020

L. Vitkov ,
W. D. Krautgartner ,
M. Hannig  and
K. Fuchs
L. Vitkov
Affiliation:
'Dental Clinic Voecklabruck of Upper-Austrian, Gebietskrankenkasse, Austria.
W. D. Krautgartner
Affiliation:
Department of Electron Microscopy, Light Microscopy and Digital Image Acquisition, Institute of Zoology, University of Salzburg, Austria.
M. Hannig
Affiliation:
Clinic of Operative Dentistry and Periodontology, University of Kiel, Germany.
K. Fuchs
Affiliation:
Institute of Pathology, Landeskrankenhaus Voecklabruck, Austria.
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Extract

The purpose of this work was to examine, by electron microscopy, the mechanism of bacterial binding to oral epithelium in patients with stomatitis using ruthenium red in combination with osmium tetroxide.

Bacteriological semi-quantitative estimation of smear from the palatal mucosa was performed in patients with stomatitis and only patients with viridans Streptococci were further included in the present investigation. Biopsies were taken from the inflamed keratinizing mucosa under regional block anesthesia in agreement with Vancouver guidelines 1978. Control samples were fixed with 1.2% glutaraldehyde (buffered at pH 6.5 with 0.1 M sodium cacodylate) for 2 h at 4° C. Postfixation was performed with 1% osmium tetroxide (buffered at pH 6.5 with 0.1 M sodium cacodylate) for 2 h at 4° C. The other samples were fixed and postfixed by the fixatives mentioned before with addition of 1.6 % ruthenium red in each case.

Type
Microorganisms: The Good, The Bad, The Unusual
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 670 - 671
Copyright
Copyright © Microscopy Society of America

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References

References:

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2.Springer, E. L. and Roth, I. L.. The ultrastructure of the capsules of Diplococcus pneumoniae and Klebsiella pneumoniae stained with ruthenium red. J Gen Microbiol 741 (1973)21.CrossRefGoogle Scholar
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