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Automated Acquisition of Cryo-Electron Microscope Images With Slow-Scan CCD Cameras and Microscope Control
Published online by Cambridge University Press: 02 July 2020
Extract
Recording electron microscope images of frozen-hydrated samples under cryo-conditions is a difficult and challenging task. The inherently low sample contrast makes accurate focusing difficult. Furthermore most frozen-hydrated samples are very beam sensitive so that electron dose on the sample must be kept to the minimum. In recent years, the new generation of cooled slow-scan CCD (SSC) cameras have shown superior properties in sensitivity, resolution, dynamic range, linearity, and more importantly their ability to display images on-line. Computer control of electron microscopes via the standard RS232C serial interface has been greatly facilitated by the use of on-line CCD images. We have developed a software package that allows cryo-electron microscope images to be automatically acquired onto a CCD camera via computer control of the microscope. This fully automated feature allows quality cryo-electron microscope images to be recorded with increased efficiency and enhances the possibility of obtaining three-dimensional reconstructions from images.
- Type
- Computational Advances and Enabling Technologies for 3D Microscopies in Biology
- Information
- Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 1113 - 1114
- Copyright
- Copyright © Microscopy Society of America 1997
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