Hostname: page-component-848d4c4894-r5zm4 Total loading time: 0 Render date: 2024-07-02T16:37:35.236Z Has data issue: false hasContentIssue false

Applications of Soft X-Ray and other Microscopy Techniques to Elucidate the Structure of Parasitic Metazoa

Published online by Cambridge University Press:  02 July 2020

W. J. Kozek
Affiliation:
Department of Microbiology and Medical Zoology, Medical Sciences Campus, University of Puerto Rico, San JuanPR
J. Brown
Affiliation:
Center for X-Ray Optics, Advanced Light Source, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
W. Meyer-Ilse
Affiliation:
Center for X-Ray Optics, Advanced Light Source, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
C. Larabell
Affiliation:
Structural Biology Laboratory, Division of Life Sciences, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
M. Moronne
Affiliation:
Structural Biology Laboratory, Division of Life Sciences, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
Get access

Extract

The small size of many parasitic organisms requires the use of election microscopy for adequate elucidation of their structure. While both transmission and scanning electron microscopy can provide complementary results which allow considerable degree of structural correlation, each technique has its inherent limitations. Since previous studies have demonstrated that soft X-ray microscopy could be used to study parasitic protozoa and provide new information, the objective of this study was to determine whether soft X-ray microscopy could also be used to elucidate the morphology of small metazoa to complement the data obtained by other microscopy techniques.

Newborn larvae, approximately 7 μm × 110 μm in size, of parasitic nematode Trichinella spiralis were used as a model system. Some of the larvae, deposited by adult females maintained in vitro, were isolated and processed for examination by transmission and scanning electron microscopy as described in our previous studies, others were fixed in 4% glutaraldehyde (Millonig's buffer) and examined in the X-ray microscope XM-1, and in the BioRad MRC 1024 confocal laser (krypton/argon) microscope of the Advanced Light Source, Berkeley National Laboratory.

Type
Biological Ultrastructure/Microbiology
Copyright
Copyright © Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Magowan, C. et al. Blood 86(1995)319CrossRefGoogle Scholar
2.Magowan, C. et al. Proc. Natl Acad Sci USA 94(1997)6222CrossRefGoogle Scholar
3.Kozek, W.J. and Orihel, T.. Int. J. Parasit. 31(1983)19.CrossRefGoogle Scholar
4. This research was supported by the Director, Office of Energy Research, Office of Basic Energy Sciences, Material Sciences Division of the U.S. Department of Energy, under contract No. DEAC03- 76SF00098, and by the ‘Research Centers in Minority Institutions’ Award RR-03051, from the National Center for Research Resources, National Institutes of Health for Research Resources, National Institutes of Health.Google Scholar