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Aging-Related Functional and Structural Changes in Renal Tissues: Lesson from a Camel Model

Published online by Cambridge University Press:  10 March 2021

Asmaa F. Khafaga Open the ORCID record for Asmaa F. Khafaga [Opens in a new window] ,
Yaser H. A. Elewa Open the ORCID record for Yaser H. A. Elewa [Opens in a new window] ,
Mustafa S. Atta  and
Ahmed E. Noreldin Open the ORCID record for Ahmed E. Noreldin [Opens in a new window]
Asmaa F. Khafaga*
Affiliation:
Pathology Department, Faculty of Veterinary Medicine, Alexandria University, Edfina22758, Egypt
Yaser H. A. Elewa
Affiliation:
Histology and Cytology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig44519, Egypt
Mustafa S. Atta
Affiliation:
Physiology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh33516, Egypt
Ahmed E. Noreldin
Affiliation:
Histology and Cytology Department, Faculty of Veterinary Medicine, Damanhour University, Damanhour22511, Egypt
*
*Author for correspondence: Asmaa F. Khafaga, E-mail: asmaa.khafaga@alexu.edu.eg
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Abstract

Renal aging is a progressive, physiological, and anatomical change that naturally occurs in all animal species. To date, no information is available concerning the aging-related structural and functional changes in camel kidneys. A total of 25 healthy male camels (14 aged 4–6 years and 11 aged 18–22 years) were included in this study. After the camels were slaughtered, samples were collected from all the camels’ kidneys and prepared for histopathological, immunohistochemical, and gene expression evaluations. The most striking observation was the significant decline in the immunohistochemical abundance of podocin and the significant upregulation of smoothening in the aging camels’ kidneys. However, the nonsignificant changes have reported for nephrin, calbindin, autophagy 5 (ATG5), aquaporin 1, and toll-like receptor 9. Furthermore, the mRNA expressions of sirtuin 1, superoxide dismutase 1, superoxide dismutase 2, peroxisome proliferator-activated receptor alpha, B-cell lymphoma 2 (Bcl-2), and erythropoietin were significantly decreased in the aging camels’ kidneys. While the significant upregulation of Bcl-2-associated X protein and the nonsignificant increase in ATG5 expression levels were reported in the aging camels’ kidneys. The present findings provide better understanding of the complex events and initiating factors of aging, allowing for the development of a future therapeutic strategy to preserve adequate renal function throughout life.

Keywords

aging camels’ kidneyaquaporin and autophagycalbindin and erythropoietinpodocin and nephrinsmoothened and toll-like receptors
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 3 , June 2021 , pp. 566 - 578
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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