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ACTH Induces ERK 1/2 Activation in Rat Adrenal Primary Cultures

Published online by Cambridge University Press:  03 October 2008

A.R. Rodrigues ,
A.M. Gouveia ,
J.G. Ferreira  and
H. Almeida
A.R. Rodrigues*
Affiliation:
Laboratório de Biologia Celular e Molecular, Faculdade de Medicina, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, andIBMC – Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal
A.M. Gouveia
Affiliation:
Laboratório de Biologia Celular e Molecular, Faculdade de Medicina, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, andIBMC – Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal Faculdade de Ciências da Nutrição e Alimentação, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal
J.G. Ferreira
Affiliation:
Laboratório de Biologia Celular e Molecular, Faculdade de Medicina, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, andIBMC – Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal
H. Almeida
Affiliation:
Laboratório de Biologia Celular e Molecular, Faculdade de Medicina, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, andIBMC – Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal
*
Corresponding author. E-mail: adrod@med.up.pt

Abstract

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Adrenocorticotropic hormone (ACTH) is the most potent stimulator of adrenal cortex, acting through the Melanocortin-2 receptor (MC2R). ACTH induces secretion of steroid hormones, critical for the normal stress response and plays also an important role on cell proliferation and differentiation. MC2R is a classical G-Protein coupled receptor (GPCR), thus activating Protein Kinase A (PKA). However, many studies suggested a cross-talk between different signalling pathways and a more complex intracellular network. In fact, in adrenocortical Y1 tumour cell line, ACTH may activate Extracellular Regulated Kinases 1/2 (ERK 1/2), which belong to the Mitogen-Activated Protein Kinases (MAPKs) family. In addition, this pathway was implicated in in vivo proliferation and steroidogenesis as shown by our group. In order to further explore and clarify ACTH signalling mechanisms, we made the present work to establish a model of primary cultures of rat adrenal cells.

Type
Abstract
Information
Microscopy and Microanalysis , Volume 14 , Supplement S3 , September 2008 , pp. 101 - 102
Copyright
Copyright © Microscopy Society of America 2008