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Migrating and Nerve Fibers-Associated Eosinophilic Granule Cells in the Olfactory Organ of Catfish (Clarias garpienius): Light and Electron Microscopic Evidence

Part of: Micrographia Collection

Published online by Cambridge University Press:  07 October 2021

Mahmoud Awad Open the ORCID record for Mahmoud Awad [Opens in a new window] ,
Walaa F.A. Emeish  and
Dalia Mohamedien Open the ORCID record for Dalia Mohamedien [Opens in a new window]
Mahmoud Awad
Affiliation:
Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena83523, Egypt
Walaa F.A. Emeish
Affiliation:
Department of Fish Diseases, Faculty of Veterinary Medicine, South Valley University, Qena83523, Egypt
Dalia Mohamedien*
Affiliation:
Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena83523, Egypt
*
*Corresponding author: Dalia Mohamedien, E-mail: daliamahmoud.histology@gmail.com
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Abstract

Piscine mast cells or eosinophilic granule cells (EGCs) of fish are equivalent to the mammalian mast cells. Recently, a better understanding of EGCs functions is allowed because of the growing interest in fish models. Herein, we present a trial to furnish data regarding the distribution of the EGCs in the fish olfactory organ, an issue that has not been reported so far. Regarding their distribution, two kinds of EGCs had been identified. An intra-epithelial one was detected in the olfactory epithelium lining of the olfactory lamellae. The stromal one was identified in the connective tissue core of the olfactory lamellae and in the lamina propria underlying the olfactory epithelium. Some were detected in the capillary lumen. The cytoplasm of the EGCs reacted strongly with the MMP-9 antibody. Stimulating a migration perspective for the olfactory EGCs which was confirmed by their location in the blood capillaries. Several EGCs could be verified in close relation, some underneath the epineurium, with the nerve fiber. Mutually, this verifies the existence of intra-epithelial and stromal migrating EGCs in the catfish olfactory organ and their inclusion in the olfactory immune response. Additionally, this provides evidence for an immune–nervous interaction to influence both the immune reactions and the nervous scheme in catfish.

Keywords

mast cellmigrationMMP-9nerve fibersolfaction
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 27 , Issue 6 , December 2021 , pp. 1582 - 1588
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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Footnotes

†

Current address: Department of Developmental Biology, Graduate School of Integrated Science for Life, Hiroshima University, Higashi-Hiroshima City 739-8526, Japan.

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