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Microscopy and Microanalysis Microscopy and Microanalysis

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Evaluating Young's Modulus of Single Yeast Cells Based on Compression Using an Atomic Force Microscope with a Flat Tip

Published online by Cambridge University Press:  15 January 2021

Di Chang [Opens in a new window] ,
Takahiro Hirate ,
Chihiro Uehara [Opens in a new window] ,
Hisataka Maruyama [Opens in a new window] ,
Nobuyuki Uozumi [Opens in a new window]  and
Fumihito Arai [Opens in a new window]
Di Chang
Affiliation:
Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Room 108, Aerospace Mechanical Engineering Research Building, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8603, Japan
Takahiro Hirate
Affiliation:
Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Room 108, Aerospace Mechanical Engineering Research Building, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8603, Japan
Chihiro Uehara
Affiliation:
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aobayama 6-6-07, Sendai 980-8579, Japan
Hisataka Maruyama
Affiliation:
Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Room 108, Aerospace Mechanical Engineering Research Building, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8603, Japan
Nobuyuki Uozumi
Affiliation:
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aobayama 6-6-07, Sendai 980-8579, Japan
Fumihito Arai
Affiliation:
Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, Room 108, Aerospace Mechanical Engineering Research Building, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8603, Japan Department of Mechanical Engineering, The University of Tokyo, Tokyo 113-8654, Japan
Corresponding
E-mail address:
changdi@biorobotics.mech.nagoya-u.ac.jp
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Abstract

In this research, atomic force microscopy (AFM) with a flat tip cantilever is utilized to measure Young's modulus of a whole yeast cell (Saccharomyces cerevisiae BY4741). The results acquired from AFM are similar to those obtained using a microfluidic chip compression system. The mechanical properties of single yeast cells are important parameters which can be examined using AFM. Conventional studies apply AFM with a sharp cantilever tip to indent the cell and measure the force-indentation curve, from which Young's modulus can be calculated. However, sharp tips introduce problems because the shape variation can lead to a different result and cannot represent the stiffness of the whole cell. It can lead to a lack of broader meaning when evaluating Young's modulus of yeast cells. In this report, we confirm the differences in results obtained when measuring the compression of a poly(dimethylsiloxane) bead using a commercial sharp tip versus a unique flat tip. The flat tip effectively avoids tip-derived errors, so we use this method to compress whole yeast cells and generate a force–deformation curve. We believe our proposed method is effective for evaluating Young's modulus of whole yeast cells.

Keywords

atomic force microscopy compression microfluidic chip yeast cell Young's modulus
Type
Original Article
Information
Microscopy and Microanalysis , First View , pp. 1 - 8
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press

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