Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Home
  • Home
Hostname: page-component-684bc48f8b-kbzls Total loading time: 0.312 Render date: 2021-04-11T12:48:32.541Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }
EnglishFrançais
Microscopy and Microanalysis Microscopy and Microanalysis

Article contents

Dilution and Magnification Effects on Image Analysis Applications in Activated Sludge Characterization

Published online by Cambridge University Press:  31 August 2010

D.P. Mesquita ,
O. Dias ,
R.A.V. Elias ,
A.L. Amaral  and
E.C. Ferreira
D.P. Mesquita
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal
O. Dias
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal
R.A.V. Elias
Affiliation:
Escola Superior de Tecnologia e de Gestão, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 134, 5301-857 Bragança, Portugal
A.L. Amaral
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal Instituto Superior de Engenharia de Coimbra, Instituto Politécnico de Coimbra, Rua Pedro Nunes, Quinta da Nora, 3030-199 Coimbra, Portugal
E.C. Ferreira
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal
Corresponding
E-mail address:
ecferreira@deb.uminho.pt
Get access
Rights & Permissions[Opens in a new window]

Abstract

The properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100× magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.

Keywords

activated sludge image analysis dilution magnification aggregates filaments
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 5 , October 2010 , pp. 561 - 568
Copyright
Copyright © Microscopy Society of America 2010

Access options

Get access to the full version of this content by using one of the access options below.
If you should have access and can't see this content please contact technical support.

References

Abreu, A.A., Costa, J.C., Araya-Kroff, P., Ferreira, E.C. & Alves, M.M. (2007). Quantitative image analysis as a diagnostic tool for identifying structural changes during a revival process of anaerobic granular sludge. Water Res 41, 1473–1480.CrossRefGoogle ScholarPubMed
Amaral, A.L. (2003). Image analysis in biotechnological processes: Applications to wastewater treatment. PhD. Thesis. Braga, Portugal: University of Minho. Available at http://hdl.handle.net/1822/4506.Google Scholar
Amaral, A.L. & Ferreira, E.C. (2005). Activated sludge monitoring of a wastewater treatment plant using image analysis and partial least squares regression. Anal Chim Acta 544, 246–253.CrossRefGoogle Scholar
Amaral, A.L., Ginoris, Y.P., Nicolau, A., Coelho, M.A.Z. & Ferreira, E.C. (2008). Stalked protozoa identification by image analysis and multivariable statistical techniques. Anal Bioanal Chem 391, 1321–1325.CrossRefGoogle ScholarPubMed
Banadda, E.N., Smets, I.Y., Jenné, R. & Van Impe, J.F. (2005). Predicting the onset filamentous bulking in biological wastewater treatment systems exploiting image analysis information. Bioproc Biosys Eng 27, 339–348.CrossRefGoogle ScholarPubMed
Bradley, J.V. (1968). Distribution-Free Statistical Tests. Englewood Cliffs, NJ: Prentice-Hall.Google Scholar
Cenens, C., Van Beurden, K.P., Jenné, R. & Van Impe, J.F. (2002). On the development of a novel image analysis technique to distinguish between flocs and filaments in activated sludge images. Water Sci Technol 46(1–2), 381–387.CrossRefGoogle ScholarPubMed
Costa, J.C., Abreu, A.A., Ferreira, E.C. & Alves, M.M. (2007). Quantitative image analysis as a diagnostic tool for monitoring structural changes of anaerobic granular sludge during detergent shock loads. Biotechnol Bioeng 98(1), 60–68.CrossRefGoogle ScholarPubMed
da Motta, M., Amaral, A.L., Neves, L., Araya-Koff, P., Ferreira, E.C., Alves, M.M., Mota, M., Roche, N., Vivier, H. & Pons, M.N. (2002). Dilution effects on biomass characterization by image analysis. In Proceedings of the 14th Brazilian Congress on Chemical Engineering, Natal, Brazil, p. 9 (CD-ROM).Google Scholar
da Motta, M., Pons, M.N. & Roche, N. (2001). Automated monitoring of activated sludge in a pilot plant using image analysis. Wat Sci Technol 43(7), 91–96.CrossRefGoogle Scholar
Ginoris, Y.P., Amaral, A.L., Nicolau, A., Coelho, M.A.Z., Ferreira, E.C. (2007a). Recognition of protozoa and metazoa using image analysis tools, discriminant analysis, neural networks and decision trees. Anal Chim Acta 595, 160–169.CrossRefGoogle ScholarPubMed
Ginoris, Y.P., Amaral, A.L., Nicolau, A., Coelho, M.A.Z. & Ferreira, E.C. (2007b). Development of an image analysis procedure for identifying protozoa and metazoa typical of activated sludge system. Water Res 41, 2581–2589.CrossRefGoogle ScholarPubMed
Jenkins, D., Richard, M.G. & Daigger, G. (2003). Manual on the Causes and Control of Activated Sludge Bulking, Foaming and Other Solids Separation Problems. Boca Raton, FL: Lewis Publishing.Google Scholar
Jenné, R., Banadda, E.N., Gins, G., Deurinck, J., Smets, I.Y., Geeraerd, A.H. & Van Impe, J.F. (2006). Use of image analysis for sludge characterisation: Studying the relation between floc shape and sludge settleability. Water Sci Technol 54(1), 167–174.CrossRefGoogle ScholarPubMed
Jenné, R., Banadda, E.N., Smets, I.Y., Deurinck, J. & Van Impe, J.F. (2007). Detection of filamentous bulking problems: Developing an image analysis system for sludge composition monitoring. Micros Microanal 13, 36–41.CrossRefGoogle ScholarPubMed
Jin, B., Wilén, B.M. & Lant, P. (2003). A comprehensive insight into floc characteristics and their impact on compressibility and settleability of activated sludge. Chem Eng J 95, 221–234.CrossRefGoogle Scholar
Mesquita, D.P., Dias, O., Amaral, A.L. & Ferreira, E.C. (2009b). Monitoring of activated sludge settling ability through image analysis: Validation on full-scale wastewater treatment plants. Bioprocess Biosyst Eng 32(3), 361–367.CrossRefGoogle ScholarPubMed
Mesquita, D.P., Dias, O., Amaral, A.L. & Ferreira, E.C. (2010). A Comparison between bright field and phase-contrast image analysis techniques in activated sludge morphological characterization. Micros Microanal 16(2), 166–174.CrossRefGoogle ScholarPubMed
Mesquita, D.P., Dias, O., Dias, A.M.A., Amaral, A.L. & Ferreira, E.C. (2009a). Correlation between sludge settling ability and image analysis information using partial least squares. Anal Chim Acta 642(1–2), 94–101.CrossRefGoogle ScholarPubMed
Wilén, B.M., Lumley, D., Mattsson, A. & Mino, T. (2008). Relationship between floc composition and flocculation and settling properties studied at a full scale activated sludge plant. Water Res 42, 4404–4418.CrossRefGoogle Scholar

Altmetric attention score

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 6
Total number of PDF views: 19 *
View data table for this chart

* Views captured on Cambridge Core between September 2016 - 11th April 2021. This data will be updated every 24 hours.

9
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Dilution and Magnification Effects on Image Analysis Applications in Activated Sludge Characterization
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Dilution and Magnification Effects on Image Analysis Applications in Activated Sludge Characterization
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Dilution and Magnification Effects on Image Analysis Applications in Activated Sludge Characterization
Available formats
×
×

Reply to: Submit a response

Your details

Conflicting interests

Do you have any conflicting interests? *