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An Automated System for Maintaining Liquid Nitrogen Levels in the Gatan Cryostage.

Published online by Cambridge University Press:  02 July 2020

S.J. Robinson
Affiliation:
Imaging Technology Group, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL61801
G. Fried
Affiliation:
Imaging Technology Group, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL61801
J. Pulokas
Affiliation:
Imaging Technology Group, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL61801

Extract

For several years we have been developing a system for the automated control and acquisition of images from a transmission electron microscope [1,2,3]. The system has been developed around a Philips CM200 equipped with a Gatan cryostage and a Gatan MSC CCD camera. One of the primary motivations in developing this software is to provide for a system that can acquire many hundreds of images over several days of completely unattended operation.

The current implementation of our automated system allows data to be automatically collected from the TEM for more than 36 hours. There is no intervention required from the operator except for one critical exception, refilling the Gatan cryostage. The cryostage, which maintains the temperature of the vitreous ice specimens, must be refilled with liquid nitrogen (LN2) approximately every two hours.

Type
Electron Cryomicroscopy of Macromolecules
Copyright
Copyright © Microscopy Society of America

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References

1.Kisseberth, N., et al. (1998) JSB 120, 309319.Google Scholar
2.Potter, C.S., et al. (1999) Ultramicroscopy, 77, 153161.CrossRefGoogle Scholar
3.Pulokas, J., et al. (1999) JSB. 128, 250256.Google Scholar
4. Support is provided by the NSF (9730056 and 9904547) and the IBM Shared University Research program.Google Scholar

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