Hostname: page-component-77c89778f8-gvh9x Total loading time: 0 Render date: 2024-07-17T14:23:10.010Z Has data issue: false hasContentIssue false
  • English
  • Français

Advances in Microwave-Assisted Processing For Electron Microscopy

Published online by Cambridge University Press:  02 July 2020

R. T. Giberson
R. T. Giberson*
Affiliation:
Research and Development, Ted Pella, Inc., Redding, CA96003
Get access

Abstract

The history of microwave-assisted processing has been dominated by the idea that microwave heating was an integral part of the equation. The separation of a microwave component from the heating effects of the radiation during sample processing has been experimentally difficult. Combined with this difficulty has been the closed cavity design of microwave ovens. This design is typical of laboratory and household ovens and results in the formation of “hot” and “cold” spots within the chamber. These spots produce regions in close proximity to each other which have widely varying heating effects on samples.

A second factor to consider with microwave heating is the effect wattage output has on rate and extent of microwave induced heating. Peak wattage outputs of all laboratory and most household microwave ovens are in excess of 650W. As a result the vast majority of all microwave-assisted protocols are based on heating parameters associated with high wattage processing.

Type
Recent Techniques for the Fixation and Staining of Biological Samples (Organized by M. Sanders and K. McDonald)
Information
Microscopy and Microanalysis , Volume 7 , Issue S2: Proceedings: Microscopy & Microanalysis 2001, Microscopy Society of America 59th Annual Meeting, Microbeam Analysis Society 35th Annual Meeting, Long Beach, California August 5-9, 2001 , August 2001 , pp. 1192 - 1193
Copyright
Copyright © Microscopy Society of America 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References:

1.Kok, L.P. and Boon, M. E.. Histochem. J. 22(1990)381.CrossRefGoogle Scholar
2.Login, G. R. and Dvorak, A. M.. Prog. Histochem. Cytochem. 27(1994)1.CrossRefGoogle Scholar
3.Login, G. R. et al., Cell Vision 3(1996)172.Google Scholar
4.Kok, L.P. et al., Scanning 15(1993)100.CrossRefGoogle Scholar
5.Giberson, R.T. et al., J. Vet. Diagn. Invest. 9(1997)61.CrossRefGoogle Scholar
6.Giberson, R. T. and Demaree, R. S., Jr. in Hajibagheri, N., Ed., Methods in Molecular Biology, Totowa, NJ Humana Press Inc. 117(1999)145.Google Scholar