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2G Alters the Utricular Macula of Chick Embryos

Published online by Cambridge University Press:  02 July 2020

Hirotaka Hara  and
César D. Fermin
Hirotaka Hara
Affiliation:
Department of Pathology. Tulane Medical School, New Orleans, La70112USA
César D. Fermin
Affiliation:
Department of Pathology. Tulane Medical School, New Orleans, La70112USA
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Extract

Chick embryos (Gallus domesticus) exposed continuously from day one until 18 days of incubation to a 2G acceleration (hyperG chicks) force hatched with altered macular epithelia. Fertile eggs were placed in an incubator and exposed to 2G on a centrifuge by the original apparatus until 18 days of incubation. Effective 2G loads were obtained by rotating the apparatus at a constant velocity of 327.5 degree/sec with the incubator mounted at 55 cm from the center axis [1]. The vestibules of the newly hatch chicks were dissected and fixed in 2.5% glutaraldehyde. The utricles were rinsed in phosphate buffer, postfixed with 2% osmic acid and dehydrated in graded ethanol and embedded in plastic. Semithin sections were examined under Light microscopy with computerized video imaging system [2] and ultrathin sections were examined under TEM.

Type
Developmental Biology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1168 - 1169
Copyright
Copyright © Microscopy Society of America

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References

1.Hara, H., et al., Acta Otolaryngol (Stockh), 1995. 519(Suppl): p. 257–261.Google Scholar
2.Fermin, C. and DeGraw, S., J. Anat, 1995.186: p. 469–481.Google Scholar
3.Fermin, C et ah, Histol Histopath, 1996.11(2): p. 407–426.Google Scholar
4. Acknowledgement, The authors thank the Dept. of Pathology at Tulane, NASA and the NIH for supporting this work..Google Scholar